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Oxidative stress in endurance cycling is reduced dose-dependently after one month of re-esterified DHA supplementation de Salazar, Lydia Torregrosa-Garcia, Antonio Luque-Rubia, Antonio J. Avila-Gandia, Vicente Domingo i Pedrol, Joan Carles López Román, Fco. Javier Estrès oxidatiu Oxidative stress Docosahexaenoic acid (DHA) supplementation can reduce exercise-induced oxidative stress generated during long aerobic exercise, with the minimum dose yet to be elucidated for physically active subjects. In this study, we performed a dose finding with re-esterified DHA in triglyceride form in a randomized double-blind parallel trial at different doses (350, 1050, 1750, and 2450 mg a day) for 4 weeks in males engaged in regular cycling (n = 100, 7.6 ± 3.7 h/week). The endogenous antioxidant capacity of DHA was quantified as a reduction in the levels of the oxidative stress marker 8-hydroxy-2'-deoxyguanosine (8-OHdG) recollected in 24-h urine samples after 90 min of constant load cycling before and after intervention. To ascertain incorporation of DHA, erythrocyte polyunsaturated fatty acid (PUFA) composition was compared along groups. We found a dose-dependent antioxidant capacity of DHA from 1050 mg with a trend to neutralization for the highest dose of 2450 mg (placebo: n = 13, F = 0.041; 350 mg: n = 10, F = 0.268; 1050 mg: n = 11, F = 7.112; 1750 mg: n = 12, F = 9.681; 2450 mg: n = 10, F = 15.230). In the erythrocyte membrane, the re-esterified DHA increased DHA and omega-3 percentage and decreased omega 6 and the omega-6 to omega-3 ratio, while Eicosapentaenoic acid (EPA) and PUFA remained unchanged. Supplementation of re-esterified DHA exerts a dose-dependent endogenous antioxidant property against moderate-intensity long-duration aerobic exercise in physically active subjects when provided at least 1050 mg a day for 4 weeks. 2020-11-30T17:52:43Z 2020-11-30T17:52:43Z 2020-11-18 2020-11-30T17:52:43Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2076-3921 https://hdl.handle.net/2445/172471 704910 33218112 eng Reproducció del document publicat a: https://doi.org/10.3390/antiox9111145 Antioxidants, 2020, vol. 9, num. 11, p. 1145 https://doi.org/10.3390/antiox9111145 cc-by (c) de Salazar, Lydia et al., 2020 http://creativecommons.org/licenses/by/3.0/es info:eu-repo/semantics/openAccess 15 p. application/pdf MDPI Articles publicats en revistes (Bioquímica i Biomedicina Molecular)