2026-04-17T01:27:39Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1721822025-12-04T19:35:38Zcom_2072_1057col_2072_478813col_2072_478917

00925njm 22002777a 4500 dc Salim, Hiba author Giménez López, Estela author Sanz Nebot, María Victoria author Benavente Moreno, Fernando J. (Julián) author 2020-11-20T09:14:37Z 2021-10-06T05:10:20Z 2020-10-06 2020-11-20T09:14:37Z The use of ionic matrices (IMs) was evaluated as an alternative to conventional matrices to analyze microRNAs (miRNAs) by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). 2, 4, 6-Trihydroxyacetophenone (THAP), 6-aza-2-thiothymine (ATT) and 3-hydroxypicolinic acid (3-HPA) and their IMs with pyridine (PYR) and butylamine (BA) were studied to analyze a standard mixture of miRNAs: miR-21, let-7g and iso-miR-16. Among all the studied matrices, ATT-PYR at 75 mg/mL in acetonitrile (MeCN):H2O (50:50, v/v) was selected as the optimal. Furthermore, addition of ammonium citrate dibasic (AC) as signal enhancer was mandatory to obtain an appropriate miRNA detection. ATT-PYR provided the best sensitivity, with limit of detection (LOD) up to 5 nM (equivalent to 1 fmol in the spot) and excellent spot-to-spot repeatability due to the improved homogeneity of the spots compared to the conventional matrices. The applicability of the established method to direct, multiplex and untargeted analysis of miRNAs in serum samples was also investigated. Espectrometria de masses Marcadors bioquímics Mass spectrometry Biochemical markers Ionic matrices for matrix-assisted laser desorption/ionization mass spectrometry analysis of microRNA biomarkers