2026-04-17T01:23:16Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1720302026-01-22T19:53:34Zcom_2072_1057col_2072_478781col_2072_478908col_2072_478917

00925njm 22002777a 4500 dc Sati, Satish author Bonev, Boyan author Szabo, Quentin author Jost, Daniel author Bensadoun, Paul author Serra, Francois author Loubiere, Vincent author Papadopoulos, Giorgio Lucio author Rivera-Mulia, Juan-Carlos author Frisch, Lauriane author Bouret, Pauline author Castillo, David author Gelpí Buchaca, Josep Lluís author Orozco López, Modesto author Vaillant, Cedric author Pellestor, Franck author Bantignies, Frederic author Martí Renom, Marc A. author Gilbert, David M. author Lemaitre, Jean-Marc author Cavalli, Giacomo author 2020-11-12T15:54:00Z 2020-11-12T15:54:00Z 2020-05-07 2020-11-12T15:54:00Z To understand the role of the extensive senescence-associated 3D genome reorganization, we generated genome-wide chromatin interaction maps, epigenome, replication-timing, whole-genome bisulfite sequencing, and gene expression profiles from cells entering replicative senescence (RS) or upon oncogene-induced senescence (OIS). We identify senescence-associated heterochromatin domains (SAHDs). Differential intra- versus inter-SAHD interactions lead to the formation of senescence-associated heterochromatin foci (SAHFs) in OIS but not in RS. This OIS-specific configuration brings active genes located in genomic regions adjacent to SAHDs in close spatial proximity and favors their expression.We also identify DNMT1 as a factor that induces SAHFs by promoting HMGA2 expression. Upon DNMT1 depletion, OIS cells transition to a 3D genome conformation akin to that of cells in replicative senescence. These data show how multi-omics and imaging can identify critical features of RS and OIS and discover determinants of acute senescence and SAHF formation. Oncogènesi Cromatina Epigenètica Carcinogenesis Chromatin Epigenetics 4D Genome rewiring during oncogene-induced and replicative senescende