2026-04-13T07:08:32Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1692502025-12-04T21:25:12Zcom_2072_1057col_2072_478779col_2072_478917

00925njm 22002777a 4500 dc Kalú Rivas, Aruani author Alcover Amengual, Maria Magdalena author Martínez-Orellana, Pamela author Montserrat-Sangrà, Sara author Nachum-Biala, Yaarit author Fisa Saladrigas, Roser author Riera Lizandra, Ma. Cristina author Baneth, Gad author Solano-Gallego, Laia author 2020-07-22T07:42:48Z 2021-06-21T05:10:21Z 2020-06-21 2020-07-22T07:42:48Z Venezuela is a country where human and canine leishmaniosis due to Leishmania infantum, Leishmania braziliensis and other Leishmania spp. is endemic. However, only limited data is available on canine Leishmania infection in Venezuela. The aim of this cross-sectional study was to evaluate the prevalence of Leishmania infection in dogs (n = 152) from the states of Lara (n = 91) and Yaracuy (n = 61) in Venezuela by means of serological and molecular methods. Physical examination was performed and blood samples were collected from all dogs. Serology for antibodies reactive with L. infantum and L. braziliensis antigens was assessed by the enzyme-linked immunosorbent assay (ELISA) and detection of Leishmania DNA from blood samples was evaluated by kinetoplast Leishmania real-time polymerase chain reaction (RT-PCR). In addition, Leishmania internal transcribed spacer (ITS-1) RT-PCR was performed on the samples positive by kinetoplast RT-PCR. The prevalence of Leishmania infection based on serological and/or molecular techniques was 11.8%. The seroprevalence for L. infantum and L. braziliensis antigens were 2.1% (3/144) and 8.3% (12/144), respectively. All dogs from the state of Yaracuy were serologically negative to L. infantum while 4.6% (4/86) of the dogs were reactive to L. braziliensis antigen. Fourteen percent (8/58) of the dogs from the state of Lara were positive to L. infantum and 5.2% (3/58) to L. braziliensis antigen. Three dogs were positive to both Leishmania spp. antigens. By RT-PCR, 6.5% (4/61) and 4.4% (4/91) of the dog were positive for infection in the states of Lara and Yaracuy, respectively. The RT-PCR product of one dog from the state of Yaracuy was sequenced revealing a 100% identity with L. infantum. However, all RT-PCR positive dogs were seronegative to both Leishmania spp. antigens. In conclusion, the positivity for Leishmania spp. infections observed indicates that dogs are frequently infected by L. infantum, L. braziliensis or related Leishmania spp. in Venezuela. Leishmaniosi Gossos Veneçuela Malalties infeccioses en els animals Leishmaniasis Dogs Venezuela Communicable diseases in animals Serological and molecular survey of Leishmania infection in dogs from Venezuela