2026-04-17T22:55:51Zhttps://recercat.cat/oai/request
oai:recercat.cat:2445/1682232025-12-05T13:16:16Zcom_2072_1057col_2072_478798col_2072_478916col_2072_478917
RECERCAT author Martinez Valenzuela, Laura author Draibe, Juliana author Quero Ramos, Maria author Fulladosa, Xavier author Cruzado, Josep Ma. author Bestard Matamoros, Oriol author Torras Ambròs, Joan 2020-07-09T11:07:56Z2020-07-09T11:07:56Z2019-11-202020-07-09T11:07:57Z Background: the role of the T helper 17 (Th17) cell subset in anti-neutrophil cytoplasm antibodies (ANCA) associated vasculitis (AAV) is controversial. We hypothesized that a specific Th17 response to myeloperoxidase (MPO) or proteinase 3 (PR3) is detectable in AAV patients and is different among the disease phases. Methods: we analyzed 43 AAV patients with renal involvement (21 acute and 22 remission patients), and 12 healthy controls. Peripheral blood mononuclear cells (PBMCs) were cultured with PR3/MPO over 48 h. Thereafter, frequencies of MPO/PR3-specific Th17 cells were assessed using an enzyme-linked immunosorbent spot (ELISpot) assay. Supernatant IL-17 concentration was quantified using ELISA. Finally, specific Th17 response after depletion of T regulatory lymphocytes (T-regs) in some remission patients was compared to the non T-reg-depleted response. Results: specific Th17 cell number was higher in acute patients compared to remission (p = 0.004). Specific Th17 cell number performed well in the disease activity detection (ROC curve area under the curve (AUC) = 0.87; p = 0.0001) with an optimal cut-off of 6 spots/million. Patients above this cut-off showed higher serum creatinine (p = 0.004), C-reactive protein (CRP) (p = 0.001) and ANCA titer (p = 0.032). Supernatant IL-17 concentration was higher in acute patients compared to remission (p = 0.035) and did not normalize to healthy control levels (p = 0.01). Conclusions: a specific Th17 cell response is present in AAV patients. This response is more pronounced in the acute phase, but persists in remission. cc-by (c) Martínez Valenzuela, Laura et al., 2019 http://creativecommons.org/licenses/by/3.0/es info:eu-repo/semantics/openAccess Vasculitis Diagnòstic Cèl·lules Immunologia Vasculitis Diagnosis Cells Immunology Exploring frequencies of circulating specific Th17 cells against myeloperoxidase and proteinase 3 in ANCA associated vasculitis info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion