2026-04-17T17:13:08Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1667042025-12-05T14:23:06Zcom_2072_1057col_2072_478816col_2072_478913col_2072_478917

00925njm 22002777a 4500 dc Barenys Espadaler, Marta author Molins Raich, Anna author Amorós-Galicia, Lola author Flick, Burkhard author Gómez Catalán, Jesús author 2020-06-26T09:22:12Z 2020-08-30T05:10:26Z 2019-08-30 2020-06-26T09:22:13Z The inclusion of a read-out to detect functional consequences of craniofacial alterations in the zebrafish embryotoxicity test will allow to evaluate these alterations which are difficult to assess morphologically, and to detect alterations in cranial nerves functions leading to impairment of jaw movements. In this study we have established an ingestion test in zebrafish larvae younger than 120 hpf. To overcome the challenge of evaluating larvae which still do not present independent feeding behaviour, we have tested the ability of 72, 96 or 102 hpf larvae to ingest food mixed with fluorescent microspheres under several conditions (dark/light, with/without shaking) to find the best experimental set-up for the test. We have included the investigation of two substances as potential positive controls: ketoconazole and tricaine. Ketoconazole 10 μM exposure during development produced significant embryotoxic effects including a characteristic craniofacial alteration pattern consisting in impaired development of brain, nasal cavity, mouth opening and jaw, as well as a significant decrease in food intake. Tricaine exposure at 380 μM during the food availability period significantly decreased the food intake. The method proposed will be a useful alternative tool to animal testing to detect compounds inducing adverse effects on craniofacial development. Peix zebra Toxicologia Embriologia Alimentació Mètodes experimentals Zebra danio Toxicology Embryology Diet Experimental methods Implementation of a functional endpoint to the zebrafish embryotoxicity test to evaluate craniofacial abnormalities