2026-04-17T07:32:26Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1665182025-12-04T21:22:20Zcom_2072_1057col_2072_478779col_2072_478917

00925njm 22002777a 4500 dc Miñana i Galbis, David author Farfán Sellarés, Maribel author Fusté Munné, M. Carme author Lorén Egea, José Gaspar author 2020-06-23T07:21:38Z 2020-06-23T07:21:38Z 2007-03 2020-06-23T07:21:38Z A polyphasic study was performed to determine the taxonomic position of two <i>Aeromonas</i> strains, 665N and 868E^T, isolated from bivalve molluscs, that could not be identified at the species level in a previous numerical taxonomy study. The DNA G+C content of these isolates was 62.3 and 62.6 mol%, respectively. Sequence analysis of the 16S rRNA gene showed that the two new strains were closely related to members of the genus <i>Aeromonas</i>. Fluorescence amplified fragment length polymorphism fingerprinting revealed that strains 665N and 868E^T clustered together with a similarity of 78% but did not cluster with any of the <i>Aeromonas</i> genomospecies. DNA-DNA hybridization experiments revealed a high level of relatedness between the two new isolates (76 %) but low levels of relatedness between these and phylogenetically most closely related <i>Aeromonas</i> genomospecies (30-44 %). Useful tests for the phenotypic differentiation of strains 665N and 868E^T from other mesophilic <i>Aeromonas</i> species included those for gas from glucose, lysine decarboxylase, Voges-Proskauer reaction, acid from L-arabinose, hydrolysis of aesculin and utilization of L-lactate. On the basis of genotypic and phenotypic evidence, strains 665N and 868ET are considered to represent a novel species of the genus <i>Aeromonas</i>, for which the name <i>Aeromonas bivalvium</i> sp. nov. is proposed. The type strain is 868E^T (=CECT 7113^T=LMG 23376^T). Microbiologia Classificació Genètica bacteriana Filogènia Taxonomia (Biologia) Microbiology Classification Bacterial genetics Phylogeny Taxonomy (Biology) Aeromonas bivalvium sp. nov. isolated from bivalve molluscs