2026-04-17T06:05:37Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1662772025-11-19T10:41:19Zcom_2072_1057col_2072_478815col_2072_478916col_2072_478917

00925njm 22002777a 4500 dc Suñé Pou, Marc author Prieto-Sánchez, Silvia author El Yousfi, Younes author Boyero-Corral, Sofía author Nardi Ricart, Anna author Nofrerias Roig, Isaac author Pérez Lozano, Pilar author García Montoya, Encarna author Miñarro Carmona, Montserrat author Ticó Grau, Josep R. author Suñé i Negre, Josep M. (Josep Maria) author Hernández-Munain, Cristina author Suñé, Carlos author 2020-06-19T06:31:47Z 2020-06-19T06:31:47Z 2018-05-30 2020-06-19T06:31:48Z Background: Cationic solid lipid nanoparticles (SLNs) have been given considerable attention for therapeutic nucleic acid delivery owing to their advantages over viral and other nanoparticle delivery systems. However, poor delivery efficiency and complex formulations hinder the clinical translation of SLNs. Aim: The aim of this study was to formulate and characterize SLNs incorporating the cholesterol derivative cholesteryl oleate to produce SLN-nucleic acid complexes with reduced cytotoxicity and more efficient cellular uptake. Methods: Five cholesteryl oleate-containing formulations were prepared. Laser diffraction and laser Doppler microelectrophoresis were used to evaluate particle size and zeta potential, respectively. Nanoparticle morphology was analyzed using electron microscopy. Cytotoxicity and cellular uptake of lipoplexes were evaluated using flow cytometry and fluorescence microscopy. The gene inhibition capacity of the lipoplexes was assessed using siRNAs to block constitutive luciferase expression. Results: We obtained nanoparticles with a mean diameter of approximately 150-200 nm in size and zeta potential values of 25-40 mV. SLN formulations with intermediate concentrations of cholesteryl oleate exhibited good stability and spherical structures with no aggregation. No cell toxicity of any reference SLN was observed. Finally, cellular uptake experiments with DNAand RNA-SLNs were performed to select one reference with superior transient transfection efficiency that significantly decreased gene activity upon siRNA complexation. Conclusion: The results indicate that cholesteryl oleate-loaded SLNs are a safe and effective platform for nonviral nucleic acid delivery. Toxicologia Nanopartícules Microscòpia Electroforesi Lípids Fluorescència Toxicology Nanoparticles Microscopy Electrophoresis Lipids Fluorescence Cholesteryl oleate-loaded cationic solid lipid nanoparticles as carriers for efficient gene-silencing therapy