2026-04-13T07:13:44Zhttps://recercat.cat/oai/requestoai:recercat.cat:2445/1652892025-12-05T14:23:44Zcom_2072_1057col_2072_478816col_2072_478917
RECERCAT
author
Teixidó Condomines, Elisabet
author
Piqué Benages, Maria Esther
author
Gómez Catalán, Jesús
author
Llobet Mallafré, Joan M. (Joan Maria)
2020-06-12T07:18:48Z2020-06-12T07:18:48Z2013-02-272020-06-12T07:18:48Z
In this study we analyzed some aspects of the assessment of developmental delay in the zebrafish embryotoxicity/teratogenicity test and explored the suitability of acetylcholinesterase (AChE) activity as a biochemical marker and as a higher throughput alternative to morphological endpoints such as head-trunk angle, tail length and morphological score. Embryos were exposed from 4 to 52 h post-fertilization (hpf) to a selection of known embryotoxic/teratogen compounds (valproic acid, retinoic acid, caffeine, sodium salicylate, glucose, hydroxyurea, methoxyacetic acid, boric acid and paraoxon-methyl) over a concentration range. They were evaluated for AChE activity, head-trunk angle, tail length and several qualitative parameters integrated in a morphological score. In general, the different patterns of the concentration-response curves allowed distinguishing between chemicals that produced growth retardation (valproic and methoxyacetic acid) and chemicals that produced non-growth-delay related malformations. An acceptable correlation between the morphological score, AChE activity and head-trunk angle as markers of developmental delay was observed, being AChE activity particularly sensitive to detect delay in the absence of malformations.
(c) Elsevier Ltd, 2013 info:eu-repo/semantics/openAccess
Enzimologia
Embriologia
Metabolisme
Acetilcolinesterasa
Nanisme
Malformacions
Peix zebra
Toxicologia
Enzymology
Embryology
Metabolism
Acetylcholinesterase
Dwarfism
Human abnormalities
Zebra danio
Toxicology
Assessment of Developmental Delay in the Zebrafish embryo Teratogenicity Assay
info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion