2026-04-17T20:18:39Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1652892025-12-05T14:23:44Zcom_2072_1057col_2072_478816col_2072_478917

urn:hdl:2445/165289 Assessment of Developmental Delay in the Zebrafish embryo Teratogenicity Assay Teixidó Condomines, Elisabet Piqué Benages, Maria Esther Gómez Catalán, Jesús Llobet Mallafré, Joan M. (Joan Maria) Enzimologia Embriologia Metabolisme Acetilcolinesterasa Nanisme Malformacions Peix zebra Toxicologia Enzymology Embryology Metabolism Acetylcholinesterase Dwarfism Human abnormalities Zebra danio Toxicology In this study we analyzed some aspects of the assessment of developmental delay in the zebrafish embryotoxicity/teratogenicity test and explored the suitability of acetylcholinesterase (AChE) activity as a biochemical marker and as a higher throughput alternative to morphological endpoints such as head-trunk angle, tail length and morphological score. Embryos were exposed from 4 to 52 h post-fertilization (hpf) to a selection of known embryotoxic/teratogen compounds (valproic acid, retinoic acid, caffeine, sodium salicylate, glucose, hydroxyurea, methoxyacetic acid, boric acid and paraoxon-methyl) over a concentration range. They were evaluated for AChE activity, head-trunk angle, tail length and several qualitative parameters integrated in a morphological score. In general, the different patterns of the concentration-response curves allowed distinguishing between chemicals that produced growth retardation (valproic and methoxyacetic acid) and chemicals that produced non-growth-delay related malformations. An acceptable correlation between the morphological score, AChE activity and head-trunk angle as markers of developmental delay was observed, being AChE activity particularly sensitive to detect delay in the absence of malformations. 2020-06-12T07:18:48Z 2020-06-12T07:18:48Z 2013-02-27 2020-06-12T07:18:48Z info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Versió postprint del document publicat a: https://doi.org/10.1016/j.tiv.2012.07.010 Toxicology in Vitro, 2013, vol. 27, num. 1, p. 469-478 https://doi.org/10.1016/j.tiv.2012.07.010 (c) Elsevier Ltd, 2013 info:eu-repo/semantics/openAccess Elsevier Ltd Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica)