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Analysis of HIV-1 Fusion Peptide inhibition by synthetic peptides from E1 protein of GB virus C Sánchez Martín, Ma. Jesús Hristova, Kalina Pujol Cubells, Montserrat Gómara Elena, María José Haro, Isabel Alsina Esteller, Ma. Asunción Busquets i Viñas, Ma. Antonia VIH (Virus) Síntesi de pèptids Hepatitis G Liposomes Microscòpia confocal HIV (Viruses) Peptide synthesis Hepatitis G Liposomes Confocal microscopy The aim of this study was to identify proteins that could inhibit the activity of the peptide sequence representing the N-terminal of the surface protein gp41 of HIV, corresponding to the fusion peptide of the virus (HIV-1 FP). To do this we synthesized and studied 58 peptides corresponding to the envelope protein E1 of the hepatitis G virus (GBV-C). Five of the E1 synthetic peptides: NCCAPEDIGFCLEGGCLV (P7), APEDIGFCLEGGCLVALG (P8), FCLEGGCL VALGCTICTD (P10), QAGLAVRPGKSAAQLVGE (P18) and AQLVGELGSLYGPLSVSA (P22) were capable of inhibiting the leakage of vesicular contents caused by HIV-1 FP. A series of experiments were carried out to determine how these E1 peptides interact with HIV-1 FP. Our studies analyzed the interactions with and without the presence of lipid membranes. Isothermal titration calorimetry revealed that the binding of P7, P18 and P22 peptides to HIV-1 FP is strongly endothermic, and that binding is entropy-driven. Gibbs energy for the process indicates a spontaneous binding between E1 peptides and HIV-1 FP. Moreover, confocal microscopy of Giant Unilamellar Vesicles revealed that the disruption of the lipid bilayer by HIV-1 FP alone was inhibited by the presence of any of the five selected peptides. Our results highlight that these E1 synthetic peptides could be involved in preventing the entry of HIV-1 by binding to the HIV-1 FP. Therefore, the continued study into the interaction between GBV-C peptides and HIV-1 FP could lead to the development of new therapeutic agents for the treatment of AIDS. 2020-06-04T15:28:12Z 2020-06-04T15:28:12Z 2011-08-01 2020-06-04T15:28:12Z info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion 0021-9797 https://hdl.handle.net/2445/164342 602037 21565353 eng Versió postprint del document publicat a: https://doi.org/10.1016/j.jcis.2011.04.053 Journal of Colloid and Interface Science, 2011, vol. 360, num. 1, p. 124-131 https://doi.org/10.1016/j.jcis.2011.04.053 (c) Elsevier, 2011 info:eu-repo/semantics/openAccess 8 p. application/pdf application/pdf Elsevier Articles publicats en revistes (Farmàcia, Tecnologia Farmacèutica i Fisicoquímica)