2026-04-13T00:04:47Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1642812025-11-19T19:08:59Zcom_2072_1057col_2072_478858col_2072_478917col_2072_478921

00925njm 22002777a 4500 dc Cruz, Tamara author López Giraldo, Alejandra author Noell, Guillaume author Casas Recasens, Sandra author Garcia, Tamara author Molins López-Rodó, Laureano author Juan, Manel author Fernandez, M.A. author Agustí García-Navarro, Àlvar author Faner, Rosa author 2020-06-04T10:58:33Z 2020-06-04T10:58:33Z 2019-07-12 2020-06-04T10:58:34Z Background: Chronic Obstructive Pulmonary Disease (COPD) is associated with an abnormal pulmonary and systemic immune response to tobacco smoking. Yet, how do immune cells relate within and between these two biological compartments, how the pulmonary infiltrate influences the lung transcriptome, and what is the role of active smoking vs. presence of disease is unclear. Methods: To investigate these questions, we simultaneously collected lung tissue and blood from 65 individuals stratified by smoking habit and presence of the disease. The immune cell composition of both tissues was assessed by flow cytometry, whole lung transcriptome was determined with Affymetrix arrays, and we used Weighted Gene Co-expression Network Analysis (WGCNA) to integrate results. Results: Main results showed that: (1) current smoking and the presence of COPD were both independently associated with a reduction in the proportion of lung T cells and an increase of macrophages, specifically those expressing CD80 + CD163+; (2) changes in the proportion of infiltrating macrophages, smoking status or the level of airflow limitation were associated to different WGCNA modules, which were enriched in iron ion transport, extracellular matrix and cilium organization gene ontologies; and, (3) circulating white blood cells counts were correlated with lung macrophages and T cells. Conclusions: Mild-moderated COPD lung immune infiltrate is associated with the active smoking status and presence of disease; is associated with changes in whole lung tissue transcriptome and marginally reflected in blood. Bronquitis Malalts crònics Hàbit de fumar Cèl·lules immunocompetents Bronchitis Chronically ill Smoking Immunocompetent cells Multi-level immune response network in mild-moderate Chronic Obstructive Pulmonary Disease (COPD)