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Biomarkers of Morbid Obesity and Prediabetes by Metabolomic Profiling of Human Discordant Phenotypes Tulipani, Sara Palau Rodríguez, Magalí Miñarro Alonso, Antonio Cardona, Fernando Marco Ramell, Anna Zonja, Bozo López de Alda, Miren Muñoz-Garach, Araceli Sànchez, Àlex (Sànchez Pla) Tinahones, Francisco J. Andrés Lacueva, Ma. Cristina Obesitat Marcadors bioquímics Diabetis no-insulinodependent Resistència a la insulina Espectrometria de masses Metabolisme Fenotip Metabolòmica Obesity Biochemical markers Non-insulin-dependent diabetes Insulin resistance Mass spectrometry Metabolism Phenotype Metabolomics Metabolomic studies aimed to dissect the connection between the development of type 2 diabetes and obesity are still scarce. In the present study, fasting serum from sixty-four adult individuals classified into four sex-matched groups by their BMI [non-obese versus morbid obese] and the increased risk of developing diabetes [prediabetic insulin resistant state versus non-prediabetic non-insulin resistant] was analyzed by LC- and FIA-ESI-MS/MS-driven metabolomic approaches. Altered levels of [lyso]glycerophospholipids was the most specific metabolic trait associated to morbid obesity, particularly lysophosphatidylcholines acylated with margaric, oleic and linoleic acids [lysoPC C17:0: R=-0.56, p=0.0003; lysoPC C18:1: R=-0.61, p=0.0001; lysoPC C18:2 R=-0.64, p<0.0001]. Several amino acids were biomarkers of risk of diabetes onset associated to obesity. For instance, glutamate significantly associated with fasting insulin [R=0.5, p=0.0019] and HOMA-IR [R=0.46, p=0.0072], while glycine showed negative associations [fasting insulin: R=-0.51, p=0.0017; HOMA-IR: R=-0.49, p=0.0033], and the branched chain amino acid valine associated to prediabetes and insulin resistance in a BMI-independent manner [fasting insulin: R=0.37, p=0.0479; HOMA-IR: R=0.37, p=0.0468]. Minority sphingolipids including specific [dihydro]ceramides and sphingomyelins also associated with the prediabetic insulin resistant state, hence deserving attention as potential targets for early diagnosis or therapeutic intervention. 2020-06-03T07:55:07Z 2020-06-03T07:55:07Z 2016-10-05 2020-06-03T07:55:07Z info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion 0009-8981 https://hdl.handle.net/2445/164099 664841 eng Versió postprint del document publicat a: https://doi.org/10.1016/j.cca.2016.10.005 Clinica Chimica Acta, 2016, vol. 463, p. 53-61 https://doi.org/10.1016/j.cca.2016.10.005 cc-by-nc-nd (c) Elsevier B.V., 2016 http://creativecommons.org/licenses/by-nc-nd/3.0/es info:eu-repo/semantics/openAccess 9 p. application/pdf Elsevier B.V. Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia)