2026-04-18T05:55:10Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1630262025-12-05T10:53:15Zcom_2072_1057col_2072_478913col_2072_478917col_2072_478924

00925njm 22002777a 4500 dc Comas Basté, Oriol author Latorre Moratalla, Mariluz author Bernacchia, Roberta author Veciana Nogués, María Teresa author Vidal Carou, Ma. Carmen author 2020-05-29T08:45:17Z 2020-05-29T08:45:17Z 2017-10-25 2020-05-29T08:45:17Z Histamine intolerance is a disorder in the homeostasis of histamine due to a reduced intestinal degradation of this amine, mainly caused by diamine oxidase (DAO) enzyme deficiency, which provokes its accumulation in plasma and the appearance of adverse health affects. A new approach for the diagnosis of this intolerance could be the determination of histamine and its metabolites in urine. The aim of this work was to develop and validate a rapid method to determine histamine and methylhistamine in human urine by Ultra High Performance Liquid Chromatography and Fluorimetric detection (UHPLC-FL). The proposed method is a consistent procedure to determine histamine and methylhistamine in less than 11 minutes with adequate linearity and sensitivity. Relative standard deviation was always lower than 5.5%, ensuring method precision; and mean recovery was greater than 99% for both analytes. The structure of histamine and methylhistamine conjugated with OPA were confirmed by UHPLC-ITD-FTMS which enabled to unequivocally identify both analytes in standards and also in urine samples. The analysis of histamine and methylhistamine in urine samples could be a potential new approach for the routine diagnosis of histamine intolerance, more patient-friendly and with clear advantages in terms of equipment and personnel demand for sample collection in comparison with current plasmatic DAO activity determination. Histamina Diamines Tolerància Cromatografia de líquids d'alta resolució Orina Humors corporals Histamine Diamines Toleration High performance liquid chromatography Urine Body fluids New approach for the diagnosis of histamine intolerance based on the determination of histamine and methylhistamine in urine.