2026-04-17T03:50:43Zhttps://recercat.cat/oai/requestoai:recercat.cat:2445/1627752025-12-05T14:11:30Zcom_2072_1057col_2072_478815col_2072_478916col_2072_478917
RECERCAT
author
Novak, Ana
author
Gutiérrez-Zamora, Mercè
author
Domenech, Lluís
author
Suñé i Negre, Josep M. (Josep Maria)
author
Miñarro Carmona, Montserrat
author
García Montoya, Encarna
author
Llop Talaverón, Josep Manuel
author
Ticó Grau, Josep R.
author
Pérez Lozano, Pilar
2020-05-28T10:38:48Z2020-05-28T10:38:48Z2018-022020-05-28T10:38:49Z
A simple analytical method for simultaneous determination of phytosterols, cholesterol and squalene in lipid emulsions was developed owing to increased interest in their clinical effects. Method development was based on commonly used stationary (C18 , C8 and phenyl) and mobile phases (mixtures of acetonitrile, methanol and water) under isocratic conditions. Differences in stationary phases resulted in peak overlapping or coelution of different peaks. The best separation of all analyzed compounds was achieved on Zorbax Eclipse XDB C8 (150 × 4.6 mm, 5 μm; Agilent) and ACN-H2 O-MeOH, 80:19.5:0.5 (v/v/v). In order to achieve a shorter time of analysis, the method was further optimized and gradient separation was established. The optimized analytical method was validated and tested for routine use in lipid emulsion analyses.
(c) Wiley, 2018 info:eu-repo/semantics/openAccess
Colesterol
Fitosterols
Metodologia
Emulsions
Lípids
Cromatografia de líquids
Cholesterol
Phytosterols
Methodology
Emulsions
Lipids
Liquid chromatography
Development and validation of a simple high-performance liquid chromatography analytical method for simultaneous determination of phytosterols, cholesterol, and squalene in parenteral lipid emulsions.
info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion