2026-04-13T07:25:04Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1615372025-12-04T21:22:22Zcom_2072_1057col_2072_478779col_2072_478909col_2072_478917

00925njm 22002777a 4500 dc Sanglas Baulenas, Ariadna author Albarral Ávila, Vicenta author Farfán Sellarés, Maribel author Lorén Egea, José Gaspar author Fusté Munné, M. Carme author 2020-05-20T07:21:13Z 2020-05-20T07:21:13Z 2016-02-13 2020-05-20T07:21:13Z tTwo hundred and twenty-one strains representative of all Aeromonas species were characterized usingthe recA gene sequence, assessing its potential as a molecular marker for the genus Aeromonas. The inter-species distance values obtained demonstrated that recA has a high discriminatory power. Phylogeneticanalysis, based on full-length gene nucleotide sequences, revealed a robust topology with clearly sepa-rated clusters for each species. The maximum likelihood tree showed the Aeromonas bestiarum strains ina well-defined cluster, containing a subset of four strains of different geographical origins in a deep inter-nal branch. Data analysis provided strong evidence of recombination at the end of the recA sequences inthese four strains. Intergenomic recombination corresponding to partial regions of the two adjacent genesrecA and recX (248 bp) was identified between A. bestiarum (major parent) and Aeromonas eucrenophila(minor parent). The low number of recombinant strains detected (1.8%) suggests that horizontal flowbetween recA sequences is relatively uncommon in this genus. Moreover, only a few nucleotide differ-ences were detected among these fragments, indicating that recombination has occurred recently. Finally,we also determined if the recombinant fragment could have influenced the structure and basic functionsof the RecA protein, comparing models reconstructed from the translated amino acid sequences of ourA. bestiarum strains with known Escherichia coli RecA structures. Genètica Models moleculars Filogènia Bacteris Conformació de proteïnes Recombinació genètica ADN recombinant Genetics Molecular models Phylogeny Bacteria Proteins conformation Genetic recombination Recombinant DNA Direct evidence of recombination in the recA gene of Aeromonas bestiarum