2026-04-17T01:02:16Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1560812025-11-19T20:55:40Zcom_2072_1057col_2072_478917col_2072_478933

00925njm 22002777a 4500 dc Álvarez, Claudio Andrés author Barriga, Andrés author Albericio Palomera, Fernando author Romero, María Soledad author Guzmán, Fanny author 2020-04-20T14:12:19Z 2020-04-20T14:12:19Z 2018-05-27 2020-04-20T14:12:20Z The elder (Sambucus spp.) tree has a number of uses in traditional medicine. Previous studies have demonstrated the antimicrobial properties of elderberry liquid extract against human pathogenic bacteria and also influenza viruses. These properties have been mainly attributed to phenolic compounds. However, other plant defense molecules, such as antimicrobial peptides (AMPs), may be present. Here, we studied peptide extracts from flowers of Sambucus nigra L. The mass spectrometry analyses determined peptides of 3 to 3.6 kDa, among them, cysteine-rich peptides were identified with antimicrobial activity against various Gram-negative bacteria, including recurrent pathogens of Chilean aquaculture. In addition, membrane blebbing on the bacterial surface after exposure to the cyclotide was visualized by SEM microscopy and SYTOX Green permeabilization assay showed the ability to disrupt the bacterial membrane. We postulate that these peptides exert their action by destroying the bacterial membrane. Medicina tradicional Agents antiinfecciosos Influenzavirus Medicaments peptídics Microorganismes patògens Traditional medicine Anti-infective agents Influenza viruses Peptide drugs Pathogenic microorganisms Identification of Antimicrobial Peptides in Flowers of Sambucus Nigra