2026-04-13T05:03:45Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1557992025-12-05T09:21:04Zcom_2072_1057col_2072_478799col_2072_478917

00925njm 22002777a 4500 dc Cao, Jiahui author Ehling, Manuel author März, Sigrid author Seebach, Jochen author Tarbashevich, Katsiaryna author Sixta, Tomas author Pitulescu, Mara E. author Werner, Ann-Cathrin author Flach, Boris author Montañez, Eloi author Raz, Erez author Adams, Ralf H. author Schnittler, Hans author 2020-04-17T11:13:59Z 2020-04-17T11:13:59Z 2017-12-20 2020-04-17T11:13:59Z VEGFR-2/Notch signalling regulates angiogenesis in part by driving the remodelling of endothelial cell junctions and by inducing cell migration. Here, we show that VEGF-induced polarized cell elongation increases cell perimeter and decreases the relative VE-cadherin concentration at junctions, triggering polarized formation of actin-driven junction-associated intermittent lamellipodia (JAIL) under control of the WASP/WAVE/ARP2/3 complex. JAIL allow formation of new VE-cadherin adhesion sites that are critical for cell migration and monolayer integrity. Whereas at the leading edge of the cell, large JAIL drive cell migration with supportive contraction, lateral junctions show small JAIL that allow relative cell movement. VEGFR-2 activation initiates cell elongation through dephosphorylation of junctional myosin light chain II, which leads to a local loss of tension to induce JAIL-mediated junctional remodelling. These events require both microtubules and polarized Rac activity. Together, we propose a model where polarized JAIL formation drives directed cell migration and junctional remodelling during sprouting angiogenesis. Metabolisme Antígens CD Cèl·lules Fisiologia Angiogènesi Factor de creixement de l'endoteli vascular Metabolism CD antigens Cells Physiology Neovascularization Vascular endothelial growth factors Polarized actin and VE-Cadherin dynamics regulate junctional remodelling and cell migration during sprouting angiogenesis