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oai:recercat.cat:2445/1528182025-11-19T19:23:58Zcom_2072_1057col_2072_478813col_2072_478917col_2072_478933

Binding thermodynamics of paromomycin, neomycin, neomycin-dinucleotide and -diPNA conjugates to bacterial and human rRNA Alguacil, Javier Robles i Brau, Jordi Ràfols Llach, Clara Bosch, Elisabeth Calorimetria Antibiòtics RNA Termodinàmica Calorimetry Antibiotics RNA Thermodynamics Isothermal titration calorimetry (ITC) is a powerful technique able to evaluate the energetics of target-drug binding within the context of drug discovery. In this work, the interactions of RNAs reproducing bacterial and human ribosomal A-site, with two well-known antibiotic aminoglycosides, Paromomycin and Neomycin, as well as several Neomycin-dinucleotide and -diPNA conjugates, have been evaluated by ITC and the corresponding thermodynamic quantities determined. The comparison of the thermodynamic data of aminoglycosides and their chemical analogues allowed to select Neomycin-diPNA conjugates as the best candidates for antimicrobial activity. 2020-03-16T10:32:23Z 2020-03-16T10:32:23Z 2016 2020-03-16T10:32:24Z info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Versió postprint del document publicat a: https://doi.org/10.1002/jmr.2513 Journal of Molecular Recognition, 2016, vol. 29, num. 4, p. 142-150 https://doi.org/10.1002/jmr.2513 (c) John Wiley & Sons, 2016 info:eu-repo/semantics/openAccess John Wiley & Sons Articles publicats en revistes (Enginyeria Química i Química Analítica)