2026-04-17T13:55:36Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1528182025-11-19T19:23:58Zcom_2072_1057col_2072_478813col_2072_478917col_2072_478933

00925njm 22002777a 4500 dc Alguacil, Javier author Robles i Brau, Jordi author Ràfols Llach, Clara author Bosch, Elisabeth author 2020-03-16T10:32:23Z 2020-03-16T10:32:23Z 2016 2020-03-16T10:32:24Z Isothermal titration calorimetry (ITC) is a powerful technique able to evaluate the energetics of target-drug binding within the context of drug discovery. In this work, the interactions of RNAs reproducing bacterial and human ribosomal A-site, with two well-known antibiotic aminoglycosides, Paromomycin and Neomycin, as well as several Neomycin-dinucleotide and -diPNA conjugates, have been evaluated by ITC and the corresponding thermodynamic quantities determined. The comparison of the thermodynamic data of aminoglycosides and their chemical analogues allowed to select Neomycin-diPNA conjugates as the best candidates for antimicrobial activity. Calorimetria Antibiòtics RNA Termodinàmica Calorimetry Antibiotics RNA Thermodynamics Binding thermodynamics of paromomycin, neomycin, neomycin-dinucleotide and -diPNA conjugates to bacterial and human rRNA