2026-04-17T18:44:56Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1527382025-11-19T21:47:45Zcom_2072_1057col_2072_478782col_2072_478917

00925njm 22002777a 4500 dc Palomo-Guerrero, Marta author Fadó Andrés, Rut author Casas, Maria author Pérez-Montero, Marta author Baena Muñoz, Miguel author Helmer, Patrick O author Domínguez, José Luis author Roig, Aina author Serra i Cucurull, Dolors author Hayen, Heiko author Stenmark, Harald author Raiborg, Camilla author Casals, Núria author 2020-03-13T12:01:36Z 2020-03-13T12:01:36Z 2019-12-23 2020-03-13T12:01:37Z Anterograde transport of late endosomes or lysosomes (LE/Lys) is crucial for proper axon growth. However, the role of energetic nutrients has been poorly explored. Malonyl-CoA is a precursor of fatty acids, and its intracellular levels highly fluctuate depending on glucose availability or the energy sensor AMP-activated protein kinase (AMPK). We demonstrate in HeLa cells that carnitine palmitoyltransferase 1C (CPT1C) senses malonyl-CoA and enhances LE/Lys anterograde transport by interacting with the endoplasmic reticulum protein protrudin and facilitating the transfer of Kinesin-1 from protrudin to LE/Lys. In cultured mouse cortical neurons, glucose deprivation, pharmacological activation of AMPK or inhibition of malonyl-CoA synthesis decreases LE/Lys abundance at the axon terminal, and shortens axon length in a CPT1C-dependent manner. These results identify CPT1C as a new regulator of anterograde LE/Lys transport in response to malonyl-CoA changes, and give insight into how axon growth is controlled by nutrients. Carnitina palmitoïl-transferasa 1 Axons Lisosomes Carnitine palmitoyltransferase I Axons Lysosomes Sensing of nutrients by CPT1C regulates late endosome/lysosome anterograde transport and axon growth