2026-04-17T17:30:00Zhttps://recercat.cat/oai/request
oai:recercat.cat:2445/1513892025-12-04T19:39:01Zcom_2072_1057col_2072_478813col_2072_478917
RECERCAT author Benavente Moreno, Fernando J. (Julián) author Peró Gascón, Roger author Pont Villanueva, Laura author Jaumot Soler, Joaquim author Barbosa Torralbo, José author Sanz Nebot, María Victoria 2020-02-27T17:24:59Z2020-12-31T06:10:18Z20182020-02-27T17:24:59Z We present capillary electrophoresis-mass spectrometry (CE-MS) in combination with advanced chemometric tools for the analysis of bioactive compounds in food, in particular for the identification of antihypertensive peptides in a nutraceutical derived from a bovine milk protein hydrolysate. Different extracts of the nutraceutical were analyzed by CE-MS, and the electropherograms were processed using a novel data analysis workflow that included regions of interest (ROIs) compression and multivariate curve resolution alternating least squares (MCR-ALS). MCR-ALS permitted the description of the nutraceutical extract as ten characteristic components with their electrophoretic profiles and mass spectra. Twenty-two compounds were tentatively identified as antihypertensive bovine casein fragments through a mass search in a database of bioactive peptides. The identity of 17 antihypertensive peptides was reliably confirmed by capillary electrophoresis-tandem mass spectrometry. The proposed analytical approach demonstrated the potential to obtain a characteristic and activity-related fingerprint for quality control and authentication of the antihypertensive nutraceutical. cc-by-nc-nd (c) Elsevier B.V., 2018 http://creativecommons.org/licenses/by-nc-nd/3.0/es info:eu-repo/semantics/openAccess Pèptids Electroforesi capil·lar Quimiometria Espectrometria de masses Peptides Capillary electrophoresis Chemometrics Mass spectrometry Identification of antihypertensive peptides in nutraceuticals by capillary electrophoresis-mass spectrometry info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion