2026-04-18T07:39:41Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1513892025-12-04T19:39:01Zcom_2072_1057col_2072_478813col_2072_478917

00925njm 22002777a 4500 dc Benavente Moreno, Fernando J. (Julián) author Peró Gascón, Roger author Pont Villanueva, Laura author Jaumot Soler, Joaquim author Barbosa Torralbo, José author Sanz Nebot, María Victoria author 2020-02-27T17:24:59Z 2020-12-31T06:10:18Z 2018 2020-02-27T17:24:59Z We present capillary electrophoresis-mass spectrometry (CE-MS) in combination with advanced chemometric tools for the analysis of bioactive compounds in food, in particular for the identification of antihypertensive peptides in a nutraceutical derived from a bovine milk protein hydrolysate. Different extracts of the nutraceutical were analyzed by CE-MS, and the electropherograms were processed using a novel data analysis workflow that included regions of interest (ROIs) compression and multivariate curve resolution alternating least squares (MCR-ALS). MCR-ALS permitted the description of the nutraceutical extract as ten characteristic components with their electrophoretic profiles and mass spectra. Twenty-two compounds were tentatively identified as antihypertensive bovine casein fragments through a mass search in a database of bioactive peptides. The identity of 17 antihypertensive peptides was reliably confirmed by capillary electrophoresis-tandem mass spectrometry. The proposed analytical approach demonstrated the potential to obtain a characteristic and activity-related fingerprint for quality control and authentication of the antihypertensive nutraceutical. Pèptids Electroforesi capil·lar Quimiometria Espectrometria de masses Peptides Capillary electrophoresis Chemometrics Mass spectrometry Identification of antihypertensive peptides in nutraceuticals by capillary electrophoresis-mass spectrometry