2026-04-14T02:58:38Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1513892025-12-04T19:39:01Zcom_2072_1057col_2072_478813col_2072_478917

urn:hdl:2445/151389 Identification of antihypertensive peptides in nutraceuticals by capillary electrophoresis-mass spectrometry Benavente Moreno, Fernando J. (Julián) Peró Gascón, Roger Pont Villanueva, Laura Jaumot Soler, Joaquim Barbosa Torralbo, José Sanz Nebot, María Victoria Pèptids Electroforesi capil·lar Quimiometria Espectrometria de masses Peptides Capillary electrophoresis Chemometrics Mass spectrometry We present capillary electrophoresis-mass spectrometry (CE-MS) in combination with advanced chemometric tools for the analysis of bioactive compounds in food, in particular for the identification of antihypertensive peptides in a nutraceutical derived from a bovine milk protein hydrolysate. Different extracts of the nutraceutical were analyzed by CE-MS, and the electropherograms were processed using a novel data analysis workflow that included regions of interest (ROIs) compression and multivariate curve resolution alternating least squares (MCR-ALS). MCR-ALS permitted the description of the nutraceutical extract as ten characteristic components with their electrophoretic profiles and mass spectra. Twenty-two compounds were tentatively identified as antihypertensive bovine casein fragments through a mass search in a database of bioactive peptides. The identity of 17 antihypertensive peptides was reliably confirmed by capillary electrophoresis-tandem mass spectrometry. The proposed analytical approach demonstrated the potential to obtain a characteristic and activity-related fingerprint for quality control and authentication of the antihypertensive nutraceutical. 2020-02-27T17:24:59Z 2020-12-31T06:10:18Z 2018 2020-02-27T17:24:59Z info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Versió postprint del document publicat a: https://doi.org/10.1016/j.chroma.2018.10.018 Journal of Chromatography A, 2018, vol. 1579, p. 129-137 https://doi.org/10.1016/j.chroma.2018.10.018 cc-by-nc-nd (c) Elsevier B.V., 2018 http://creativecommons.org/licenses/by-nc-nd/3.0/es info:eu-repo/semantics/openAccess Elsevier B.V. Articles publicats en revistes (Enginyeria Química i Química Analítica)