2026-04-17T16:16:23Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1510472025-12-04T22:44:32Zcom_2072_1057col_2072_478825col_2072_478917

00925njm 22002777a 4500 dc Gómez-Gómez, Clara author Blanco-Picazo, Pedro author Brown-Jaque, Maryury author Quirós Fernández, Pablo author Rodríguez-Rubio, Lorena author Cerdà Cuéllar, Marta author Muniesa Pérez, Ma. Teresa author 2020-02-24T17:04:07Z 2020-02-24T17:04:07Z 2019-09-16 2020-02-24T17:04:07Z Bacteriophages can package part of their host's genetic material, including antibiotic resistance genes (ARGs), contributing to a rapid dissemination of resistances among bacteria. Phage particles containing ARGs were evaluated in meat, pork, beef and chicken minced meat, and ham and mortadella, purchased in local retailer. Ten ARGs (blaTEM, blaCTX-M-1, blaCTX-M-9, blaOXA-48, blaVIM, qnrA, qnrS, mecA, armA and sul1) were analyzed by qPCR in the phage DNA fraction. The genes were quantified, before and after propagation experiments in Escherichia coli, to evaluate the ability of ARG-carrying phage particles to infect and propagate in a bacterial host. According to microbiological parameters, all samples were acceptable for consumption. ARGs were detected in most of the samples after particle propagation indicating that at least part of the isolated phage particles were infectious, being sul1the most abundant ARG in all the matrices followed by β-lactamase genes. ARGs were also found in the phage DNA fraction of thirty-seven archive chicken cecal samples, confirming chicken fecal microbiota as an important ARG reservoir and the plausible origin of the particles found in meat. Phages are vehicles for gene transmission in meat that should not be underestimated as a risk factor in the global crisis of antibiotic resistance. Bacteriòfags Seguretat alimentària Bacteriophages Food security Infectious phage particles packaging antibiotic resistance genes found in meat products and chicken feces