2026-04-14T03:19:59Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1509652025-12-05T05:44:29Zcom_2072_1057col_2072_478824col_2072_478858col_2072_478917col_2072_478921

Assessment of a Loop-Mediated Isothermal Amplification (LAMP) Assay for the Rapid Detection of Pathogenic Bacteria from Respiratory Samples in Patients with Hospital-Acquired Pneumonia Vergara, Andrea Boutal, Hervé Ceccato, Adrian López, Miriam Cruells, Adrián Bueno, Leticia Moreno-Morales, Javier Puig de la Bellacasa, Jordi Castro, Pedro Torres Martí, Antoni Marco Reverté, Francesc Casals Pascual, Climent Vila Estapé, Jordi Diagnòstic Medicina intensiva Aparell respiratori Pneumònia adquirida a la comunitat Diagnosis Critical care medicine Respiratory organs Community-acquired pneumonia Rapid identification of the causative agent of hospital-acquired pneumonia (HAP) will allowan earlier administration of a more appropriate antibiotic and could improve the outcome of thesepatients. The aim of this study was to develop a rapid protocol to identify the main microorganismsinvolved in HAP by loop-mediated isothermal amplification (LAMP) directly from respiratory samples.First of all, a rapid procedure (<30 min) to extract the DNA from bronchoalveolar lavage (BAL),endotracheal aspirate (EA) or bronchoaspirate (BAS) was set up. A specific LAMP forStaphylococcusaureus,Escherichia coli,Klebsiella pneumoniae, Pseudomonas aeruginosa,Stenotrophomonas maltophiliaandAcinetobacter baumanniiwas performed with the extracted solution at 65◦C for 30-40 min. Overall,58 positive BAL and 83 EA/BAS samples were tested. The limits of detection varied according to themicroorganism detected. Validation of the LAMP assay with BAL samples showed that the assay was 100% specific and 86.3% sensitive (positive predictive value of 100% and a negative predictive valueof 50%) compared with culture. Meanwhile for BAS/EA samples, the assay rendered the followingstatistical parameters: 100% specificity, 94.6% sensitivity, 100% positive predictive value and 69.2%negative predictive value. The turnaround time including sample preparation and LAMP was circa1 h. LAMP method may be used to detect the most frequent bacteria causing HAP. It is a simple,cheap, sensitive, specific and rapid assay. 2020-02-21T12:16:25Z 2020-02-21T12:16:25Z 2020-01-11 2020-02-21T12:16:25Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2076-2607 https://hdl.handle.net/2445/150965 695192 31940771 eng Reproducció del document publicat a: https://doi.org/10.3390/microorganisms8010103 Microorganisms, 2020, vol. 8, num. 1, p. 103 https://doi.org/10.3390/microorganisms8010103 cc-by (c) Vergara, Andrea et al., 2020 http://creativecommons.org/licenses/by/3.0/es info:eu-repo/semantics/openAccess 10 p. application/pdf MDPI Articles publicats en revistes (Fonaments Clínics)