2026-04-14T09:30:54Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1433322025-12-10T00:59:46Zcom_2072_1057col_2072_478917

A Plasmodium falciparum C-mannosyltransferase is dispensable for parasite asexual blood stage development López Gutiérrez, Borja Cova, Marta Izquierdo Lázaro, Luis Plasmodium falciparum Paràsits Parasites C-mannosylation was recently identified in the thrombospondin-related anonymous protein (TRAP) from Plasmodium falciparum salivary gland sporozoites. A candidate P. falciparum C-mannosyltransferase (Pf DPY-19) was demonstrated to modify thrombospondin type 1 repeat (TSR) domains in vitro, exhibiting a different acceptor specificity than their mammalian counterparts. According to the described minimal acceptor of Pf DPY19, several TSR domain-containing proteins of P. falciparum could be C-mannosylated in vivo. However, the relevance of this protein modification for the parasite viability remains unknown. In the present study, we used CRISPR/Cas9 technology to generate a Pf DPY19 null mutant, demonstrating that this glycosyltransferase is not essential for the asexual blood development of the parasite. Pf DPY19 gene disruption was not associated with a growth phenotype, not even under endoplasmic reticulum-stressing conditions that could impair protein folding. The data presented in this work strongly suggest that Pf DPY19 is unlikely to play a critical role in the asexual blood stages of the parasite, at least under in vitro conditions. 2019-10-29T11:23:53Z 2019-10-29T11:23:53Z 2019-09-27 2019-10-25T18:00:30Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 0031-1820 https://hdl.handle.net/2445/143332 31559936 eng Reproducció del document publicat a: http://dx.doi.org/10.1017/S0031182019001380 Parasitology, 2019 http://dx.doi.org/10.1017/S0031182019001380 cc by (c) Cambridge University Press, 2019 http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess 6 p. application/pdf Cambridge University Press Articles publicats en revistes (ISGlobal)