2026-04-13T01:24:21Zhttps://recercat.cat/oai/requestoai:recercat.cat:2445/1349382025-12-04T19:38:47Zcom_2072_1057col_2072_478813col_2072_478917
RECERCAT
author
Barbosa Torralbo, José
author
Sanz Nebot, María Victoria
author
Benavente Moreno, Fernando J. (Julián)
author
Giménez López, Estela
2019-06-12T10:11:28Z2019-06-12T10:11:28Z2006-06-012019-06-12T10:11:28Z
Glycosylation is the most common posttranslational modification in proteins and the carbohydrates participate in many biological processes. The number and type of glycoforms for a certain glycoprotein may change as a consequence of pathological processes. In our work a method for the separation of transferrin sialoforms has been developed, that permits the diagnostic of Congenital Disorders of Glycosylation (CDG) using a polybrene-dextran sulphate coating and CE-ESI-TOF methodologies. In order to improve the sensitivity the use of solid-phase extraction coupled on-line to CE-ESI-MS is studied and the SPE-CE-ESI-MS developed methods are applied for the characterization of rHuEPO glycoforms. The achieved separation and the high mass-resolving power of flight (TOF) mass detection allows to establish the most probable rHuEPO glycoforms.
, 2006 info:eu-repo/semantics/openAccess
Electroforesi capil·lar
Glicoproteïnes
Espectrometria de masses
Proteïnes
Capillary electrophoresis
Glycoproteins
Mass spectrometry
Proteins
Characterization of glycoproteins by capillary electrophoresis electrospray mass spectrometry (CE-ESI-MS). Applications to diagnosis in biomedicine
info:eu-repo/semantics/article