2026-04-17T03:01:22Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1349382025-12-04T19:38:47Zcom_2072_1057col_2072_478813col_2072_478917

00925njm 22002777a 4500 dc Barbosa Torralbo, José author Sanz Nebot, María Victoria author Benavente Moreno, Fernando J. (Julián) author Giménez López, Estela author 2019-06-12T10:11:28Z 2019-06-12T10:11:28Z 2006-06-01 2019-06-12T10:11:28Z Glycosylation is the most common posttranslational modification in proteins and the carbohydrates participate in many biological processes. The number and type of glycoforms for a certain glycoprotein may change as a consequence of pathological processes. In our work a method for the separation of transferrin sialoforms has been developed, that permits the diagnostic of Congenital Disorders of Glycosylation (CDG) using a polybrene-dextran sulphate coating and CE-ESI-TOF methodologies. In order to improve the sensitivity the use of solid-phase extraction coupled on-line to CE-ESI-MS is studied and the SPE-CE-ESI-MS developed methods are applied for the characterization of rHuEPO glycoforms. The achieved separation and the high mass-resolving power of flight (TOF) mass detection allows to establish the most probable rHuEPO glycoforms. Electroforesi capil·lar Glicoproteïnes Espectrometria de masses Proteïnes Capillary electrophoresis Glycoproteins Mass spectrometry Proteins Characterization of glycoproteins by capillary electrophoresis electrospray mass spectrometry (CE-ESI-MS). Applications to diagnosis in biomedicine