2026-04-13T00:30:18Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1344982025-12-04T20:45:38Zcom_2072_1057col_2072_478822col_2072_478917

00925njm 22002777a 4500 dc Sorkin, Raya author Bergamaschi, Giulia author Kansma, Douwe author Brand, Guy author Dekel, Elya author Ofir Birin, Yifat author Rudik, Ariel author Gironella, Marta author Ritort Farran, Fèlix author Regev Rudzki, Neta author Roos, Wouter H. author Wuite, Gijs J. L. author 2019-06-04T11:54:38Z 2019-06-04T11:54:38Z 2018-08 2019-06-04T11:54:38Z A large number of studies demonstrate that cell mechanics and pathology are intimately linked. In particular, deformability of red blood cells (RBCs) is key to their function and is dramatically altered in the time course of diseases such as anemia and malaria. Due to the physiological importance of cell mechanics, many methods for cell mechanical probing have been developed. While single-cell methods provide very valuable information, they are often technically challenging and lack the high data throughput needed to distinguish differences in heterogeneous populations, while fluid-flow high-throughput methods miss the accuracy to detect subtle differences. Here we present a new method for multiplexed singlecell mechanical probing using acoustic force spectroscopy (AFS). We demonstrate that mechanical differences induced by chemical treatments of known effect can be measured and quantified. Furthermore, we explore the effect of extracellular vesicles (EVs) uptake on RBC mechanics and demonstrate that EVs uptake increases RBC deformability. Our findings demonstrate the ability of AFS to manipulate cells with high stability and precision and pave the way to further new insights into cellular mechanics and mechanobiology in health and disease, as well as potential biomedical applications. Acústica Fisiologia cel·lular Biomecànica Acoustics Cell physiology Biomechanics Probing cellular mechanics with acoustic force spectroscopy