2026-04-17T00:44:52Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1339412025-12-04T21:14:32Zcom_2072_1057col_2072_478781col_2072_478908col_2072_478917col_2072_478933

00925njm 22002777a 4500 dc Terrazas Martínez, Montserrat author Genna, Vito author Portella, Guillem author Villegas, Núria author Sánchez, Dani author Arnan, Carme author Pulido Quetglas, Carlos author Johnson, Rory author Guigó Serra, Roderic author Brun Heath, Isabelle author Aviñó Andrés, Anna author Eritja i Casadellà, Ramon author Orozco López, Modesto author 2019-05-27T09:57:28Z 2020-04-25T05:10:16Z 2019-04-25 2019-05-23T12:56:10Z We analyze the physical origin and the chemical and biological consequences of the asymmetry that occurs in DNA·RNA hybrids when the purine/pyrimidine (Pu/Py) ratio is different in the DNA and RNA strands. When the DNA strand of the hybrid is Py rich, the duplex is much more stable, rigid, and A-like than when the DNA strand is Pu rich. The origins of this dramatic asymmetry are double: first, the apparently innocuous substitution dT → rU produces a significant decrease in stacking, and second, backbone distortions are larger for DNA(Pu)·RNA(Py) hybrids than for the mirror RNA(Pu)·DNA(Py) ones. The functional impact of the structural and dynamic asymmetry in the biological activities of hybrids is dramatic and can be used to improve the efficiency of antisense-type strategies on the basis of the degradation of hybrids by RNase H or gene editing using CRISPR-Cas9 technology. Estereoquímica RNA ADN Stereochemistry RNA DNA The Origins and the Biological Consequences of the Pur/Pyr DNA·RNA Asymmetry