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A high-fat high-sucrose diet affects the long-term metabolic fate of grape proanthocyanidins in rats Molinar Toribio, Eunice María Fuguet i Jordà, Elisabet Ramos Romero, Sara Taltavull, Núria Méndez, Lucía Nogués, Maria Rosa Medina, Isabel Torres Simón, Josep Lluís Pérez-Jiménez, Jara Polifenols Metabòlits Dieta Biodisponibilitat Polyphenols Metabolites Diet Bioavailability Purpose Polyphenol metabolites are key mediators of the biological activities of polyphenols. This study aimed to evaluate the long-term effects of a high-fat high-sucrose (HFHS) diet on the metabolism of proanthocyanidins from grape seed extract (GSE). Methods Adult female Wistar-Kyoto rats were fed a standard (STD) or HFHS diet supplemented or not with GSE for 16 weeks. PA metabolites were determined by targeted HPLC-MS/MS analysis. Results A lower concentration of total microbial-derived PA metabolites was present in urine and the aqueous fraction of faeces in the HFHS + GSE group than in the STD + GSE group. In contrast, a tendency towards the formation of conjugated (epi)catechin metabolites in the HFHS + GSE group was observed. Conclusions These results show that a HFHS diet significantly modifies PA metabolism, probably via: (1) a shift in microbial communities not counteracted by the polyphenols themselves; and (2) an up-regulation of hepatic enzymes. 2019-05-21T12:44:58Z 2019-05-21T12:44:58Z 2018-02 2019-05-21T12:44:58Z info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Versió postprint del document publicat a: https://doi.org/10.1007/s00394-016-1323-9 European Journal of Nutrition, 2018, vol. 57, p. 339-349 https://doi.org/10.1007/s00394-016-1323-9 (c) Springer Verlag, 2018 info:eu-repo/semantics/openAccess Springer Verlag Articles publicats en revistes (Enginyeria Química i Química Analítica)