2026-04-17T05:56:29Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1335982025-12-04T19:36:17Zcom_2072_1057col_2072_478813col_2072_478917

00925njm 22002777a 4500 dc Molinar Toribio, Eunice María author Fuguet i Jordà, Elisabet author Ramos Romero, Sara author Taltavull, Núria author Méndez, Lucía author Nogués, Maria Rosa author Medina, Isabel author Torres Simón, Josep Lluís author Pérez-Jiménez, Jara author 2019-05-21T12:44:58Z 2019-05-21T12:44:58Z 2018-02 2019-05-21T12:44:58Z Purpose Polyphenol metabolites are key mediators of the biological activities of polyphenols. This study aimed to evaluate the long-term effects of a high-fat high-sucrose (HFHS) diet on the metabolism of proanthocyanidins from grape seed extract (GSE). Methods Adult female Wistar-Kyoto rats were fed a standard (STD) or HFHS diet supplemented or not with GSE for 16 weeks. PA metabolites were determined by targeted HPLC-MS/MS analysis. Results A lower concentration of total microbial-derived PA metabolites was present in urine and the aqueous fraction of faeces in the HFHS + GSE group than in the STD + GSE group. In contrast, a tendency towards the formation of conjugated (epi)catechin metabolites in the HFHS + GSE group was observed. Conclusions These results show that a HFHS diet significantly modifies PA metabolism, probably via: (1) a shift in microbial communities not counteracted by the polyphenols themselves; and (2) an up-regulation of hepatic enzymes. Polifenols Metabòlits Dieta Biodisponibilitat Polyphenols Metabolites Diet Bioavailability A high-fat high-sucrose diet affects the long-term metabolic fate of grape proanthocyanidins in rats