2026-04-17T02:38:42Zhttps://recercat.cat/oai/request
oai:recercat.cat:2445/1268692025-11-20T15:07:57Zcom_2072_1057col_2072_478916col_2072_478917
RECERCAT author Sánchez Velázquez, Patricia author Castellví, Quim author Villanueva Garatachea, Alberto author Quesada, Rita author Pañella, Clara author Cáceres, M. author Dorcaratto, D. author Andaluz, Anna author Moll, Xavier author Trujillo, M. author Burdío, J. M. author Berjano, E. author Grande, L. author Ivorra, Antoni author Burdío, Fernando 2018-12-11T10:22:33Z2018-12-11T10:22:33Z2016-04-012018-07-25T07:51:01Z Irreversible electroporation is a fast-growing liver ablation technique. Although safety has been well documented in small ablations, our aim is to assess its safety and feasibility when a large portion of liver is ablated. Eighty-seven mice were subjected to high voltage pulses directly delivered across parallel plate electrodes comprising around 40% of mouse liver. One group consisted in 55 athymicnude, in which a tumor from the KM12C cell line was grown and the other thirty-two C57-Bl6 nontumoral mice. Both groups were subsequently divided into subsets according to the delivered field strength (1000 V/cm, 2000 V/cm) and whether or not they received anti-hyperkalemia therapy. Early mortality (less than 24 hours post-IRE) in the 2000 V/cm group was observed and revealed considerably higher mean potassium levels. In contrast, the animals subjected to a 2000 V/cm field treated with the anti-hyperkalemia therapy had higher survival rates (OR = 0.1, 95%CI = 0.02-0.32, p < 0.001). Early mortality also depended on the electric field magnitude of the IRE protocol, as mice given 1000 V/cm survived longer than those given 2000 V/cm (OR = 4.7, 95% CI = 1.8-11.8, p = 0.001). Our findings suggest that ionic disturbances, mainly due to potassium alterations, should be warned and envisioned when large volume ablations are performed by IRE. cc by (c) Sánchez Velázquez et al., 2016 http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Mortalitat Malalties del fetge Mortality Liver diseases Irreversible electroporation of the liver: is there a safe limit to the ablation volume? info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion