2026-04-13T00:04:00Zhttps://recercat.cat/oai/request
oai:recercat.cat:2445/1253642025-12-05T12:18:47Zcom_2072_1057col_2072_478916col_2072_478917col_2072_478929
RECERCAT author Schmitz, Matthias author Llorens Torres, Franc author Pracht, Alexander author Thom, Tobias author Correia, Ângela author Zafar, Saima author Ferrer, Isidro (Ferrer Abizanda) author Zerr, Inga 2018-10-16T13:23:15Z2018-10-16T13:23:15Z2016-11-012018-07-24T12:15:50Z The identification of reliable diagnostic biomarkers in differential diagnosis of neurodegenerative diseases is an ongoing topic. A previous two-dimensional proteomic study on cerebrospinal fluid (CSF) revealed an elevated level of an enzyme, mitochondria! malate dehydrogenase 1 (MDH1), in sporadic Creutzfeldt-Jakob disease (sCJD) patients. Here, we could demonstrate the expression of MDH1 in neurons as well as in the neuropil. Its levels are lower in sCJD brains than in control brains. An examination of CSF-MDH1 in sCJD patients by ELISA revealed a significant elevation of CSF-MDH1 levels in sCJD patients (independently from the PRNP codon 129 MV genotype or the prion protein scrapie (PrPsc) type) in comparison to controls. In combination with total tau (tau), CSF-MDH1 detection exhibited a high diagnostic accuracy for sCJD diagnosis with a sensitivity of 97.5% and a specificity of 95.6%. A correlation study of MDH1 level in CSF with other neurodegenerative marker proteins revealed a significant positive correlation between MDH1 concentration with tau, 14-3-3 and neuron specific enolase level. In conclusion, our study indicated the potential of MDH1 in combination with tau as an additional biomarker in sCJD improving diagnostic accuracy of tau markedly. cc-by (c) Schmitzet et al., 2016 http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Malaltia de Creutzfeldt-Jakob Marcadors bioquímics Creutzfeldt-Jakob disease Biochemical markers Regulation of human cerebrospinal fluid malate dehydrogenase 1 in sporadic Creutzfeldt-Jakob disease patients info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion