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Cholesterol depletion in adipocytes causes caveolae collapse concomitant with proteosomal degradation of cavin-2 in a switch-like fashion Breen, Michael P., 1940- Camps Camprubí, Marta Carvalho-Simoes, Francisco Zorzano Olarte, Antonio Pilch, Paul F. Colesterol Proteïnes de membrana Cèl·lules animals Cholesterol Membrane proteins Animal cells Caveolae, little caves of cell surfaces, are enriched in cholesterol, a certain level of which is required for their structural integrity. Here we show in adipocytes that cavin-2, a peripheral membrane protein and one of 3 cavin isoforms present in caveolae from non-muscle tissue, is degraded upon cholesterol depletion in a rapid fashion resulting in collapse of caveolae. We exposed 3T3-L1 adipocytes to the cholesterol depleting agent methyl-β-cyclodextrin, which results in a sudden and extensive degradation of cavin-2 by the proteasome and a concomitant movement of cavin-1 from the plasma membrane to the cytosol along with loss of caveolae. The recovery of cavin-2 at the plasma membrane is cholesterol-dependent and is required for the return of cavin-1 from the cytosol to the cell surface and caveolae restoration. Expression of shRNA directed against cavin-2 also results in a cytosolic distribution of cavin-1 and loss of caveolae. Taken together, these data demonstrate that cavin-2 functions as a cholesterol responsive component of caveolae that is required for cavin-1 localization to the plasma membrane, and caveolae structural integrity. 2018-09-17T13:11:26Z 2018-09-17T13:11:26Z 2012-04-06 2018-09-17T13:11:26Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0034516 PLoS One, 2012, vol. 7, num. 4, p. 1-8 https://doi.org/10.1371/journal.pone.0034516 cc-by (c) Breen, Michael P., 1940- et al., 2012 http://creativecommons.org/licenses/by/3.0/es info:eu-repo/semantics/openAccess Public Library of Science (PLoS) Articles publicats en revistes (Bioquímica i Biomedicina Molecular)