2026-04-17T05:18:25Zhttps://recercat.cat/oai/request
oai:recercat.cat:2445/1246282025-12-04T21:14:20Zcom_2072_1057col_2072_478781col_2072_478917
RECERCAT author Breen, Michael P., 1940- author Camps Camprubí, Marta author Carvalho-Simoes, Francisco author Zorzano Olarte, Antonio author Pilch, Paul F. 2018-09-17T13:11:26Z2018-09-17T13:11:26Z2012-04-062018-09-17T13:11:26Z Caveolae, little caves of cell surfaces, are enriched in cholesterol, a certain level of which is required for their structural integrity. Here we show in adipocytes that cavin-2, a peripheral membrane protein and one of 3 cavin isoforms present in caveolae from non-muscle tissue, is degraded upon cholesterol depletion in a rapid fashion resulting in collapse of caveolae. We exposed 3T3-L1 adipocytes to the cholesterol depleting agent methyl-β-cyclodextrin, which results in a sudden and extensive degradation of cavin-2 by the proteasome and a concomitant movement of cavin-1 from the plasma membrane to the cytosol along with loss of caveolae. The recovery of cavin-2 at the plasma membrane is cholesterol-dependent and is required for the return of cavin-1 from the cytosol to the cell surface and caveolae restoration. Expression of shRNA directed against cavin-2 also results in a cytosolic distribution of cavin-1 and loss of caveolae. Taken together, these data demonstrate that cavin-2 functions as a cholesterol responsive component of caveolae that is required for cavin-1 localization to the plasma membrane, and caveolae structural integrity. cc-by (c) Breen, Michael P., 1940- et al., 2012 http://creativecommons.org/licenses/by/3.0/es info:eu-repo/semantics/openAccess Colesterol Proteïnes de membrana Cèl·lules animals Cholesterol Membrane proteins Animal cells Cholesterol depletion in adipocytes causes caveolae collapse concomitant with proteosomal degradation of cavin-2 in a switch-like fashion info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion