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E-cadherin: A determinant molecule associated with ovarian cancer progression, dissemination and aggressiveness Rosso, Marina Majem, Blanca Devis, Laura Lapyckyj, Lara Besso, María José Llauradó, Marta Abascal, María Florencia Matos, María Laura Lanau, Lucia Castellví, Josep Sánchez, José Luis Pérez Benavente, Asunción Gil Moreno, Antonio Reventós Puigjaner, Jaume Santamaria Margalef, Anna Rigau, Marina Hebe Vazquez Levin, Mónica Càncer d'ovari Ascites Expressió gènica Ovarian cancer Gene expression Ovarian cancer (OC) is the fifth cancer death cause in women worldwide. The malignant nature of this disease stems from its unique dissemination pattern. Epithelial-to-mesenchymal transition (EMT) has been reported in OC and downregulation of Epithelial cadherin (E-cadherin) is a hallmark of this process. However, findings on the relationship between E-cadherin levels and OC progression, dissemination and aggressiveness are controversial. In this study, the evaluation of E-cadherin expression in an OC tissue microarray revealed its prognostic value to discriminate between advanced-and early-stage tumors, as well as serous tumors from other histologies. Moreover, E-cadherin, Neural cadherin (N-cadherin), cytokeratins and vimentin expression was assessed in TOV-112, SKOV-3, OAW-42 and OV-90 OC cell lines grown in monolayers and under anchorage-independent conditions to mimic ovarian tumor cell dissemination, and results were associated with cell aggressiveness. According to these EMT-related markers, cell lines were classified as mesenchymal (M; TOV-112), intermediate mesenchymal (IM; SKOV-3), intermediate epithelial (IE; OAW-42) and epithelial (E; OV-90). M-and IM-cells depicted the highest migration capacity when grown in monolayers, and aggregates derived from M-and IM-cell lines showed lower cell death, higher adhesion to extracellular matrices and higher invasion capacity than E- antigen 125 levels more than 500 U/mL and platinum-free intervals less than 6 months. Altogether, E-cadherin expression levels were found relevant for the assessment of OC progression and aggressiveness.and IE-aggregates. The analysis of E-cadherin, N-cadherin, cytokeratin 19 and vimentin mRNA levels in 20 advanced-stage high-grade serous human OC ascites showed an IM phenotype in all cases, characterized by higher proportions of N-to E-cadherin and vimentin to cytokeratin 19. In particular, higher E-cadherin mRNA levels were associated with cancer antigen 125 levels more than 500 U/mL and platinum-free intervals less than 6 months. Altogether, E-cadherin expression levels were found relevant for the assessment of OC progression and aggressiveness. 2018-09-04T13:59:36Z 2018-09-04T13:59:36Z 2017-09-21 2018-07-24T12:00:59Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0184439 PLos One, 2017, vol. 12, num. 9, p. e0184439 https://doi.org/10.1371/journal.pone.0184439 info:eu-repo/grantAgreement/EC/FP7/269285/EU//PROTBIOFLUID cc by (c) Rosso et al., 2017 http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Public Library of Science (PLoS) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))