2026-04-17T01:05:43Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1240452025-11-20T13:50:16Zcom_2072_1057col_2072_478799col_2072_478916col_2072_478917

00925njm 22002777a 4500 dc Michailidi, Christina author Soudry, Ethan author Brait, Mariana author Maldonado, Leonel author Jaffe, Andrew author Ili-Gangas, Carmen author Brebi-Mieville, Priscilla author Perez, Jimena author Kim, Myoung Sook author Zhong, Xiaoli author Yang, Quiang author Valle, Blanca author Meltzer, Stephen J. author Torbenson, Michael author Esteller, Manel author Sidransky, David author Rafael, Guerrero-Preston author 2018-07-30T09:18:00Z 2018-07-30T09:18:00Z 2014-04-15 2018-07-30T09:18:01Z The majority of the epigenomic reports in hepatocellular carcinoma have focused on identifying novel differentially methylated drivers or passengers of the oncogenic process. Few reports have considered the technologies in place for clinical translation of newly identified biomarkers. The aim of this study was to identify epigenomic technologies that need only a small number of samples to discriminate HCC from non-HCC tissue, a basic requirement for biomarker development trials. To assess that potential, we used quantitative Methylation Specific PCR, oligonucleotide tiling arrays, and Methylation BeadChip assays. Concurrent global DNA hypomethylation, gene-specific hypermethylation, and chromatin alterations were observed as a hallmark of HCC. A global loss of promoter methylation was observed in HCC with the Illumina BeadChip assays and the Nimblegen oligonucleotide arrays. HCC samples had lower median methylation peak scores and a reduced number of significant promoter-wide methylated probes. Promoter hypermethylation of RASSF1A, SSBP2, and B4GALT1 quantified by qMSP had a sensitivity ranging from 38% to 52%, a specificity of 100%, and an AUC from 0.58 to 0.75. A panel combining these genes with HCC risk factors had a sensitivity of 87%, a specificity of 100%, and an AUC of 0.91. Epigenètica Cèl·lules hepàtiques Marcadors bioquímics Càncer de fetge Metilació Epigenetics Liver cells Biochemical markers Liver cancer Methylation Genome-wide and gene-specific epigenomic platforms for hepatocellular carcinoma biomarker development trials