2026-04-14T02:27:00Zhttps://recercat.cat/oai/request
oai:recercat.cat:2445/1240172025-12-05T09:23:52Zcom_2072_1057col_2072_478799col_2072_478916col_2072_478917
RECERCAT author Ramalho-Carvalho, João author Gonçalves, Céline S author Graça, Inês author Bidarra, David author Pereira-Silva, Eva author Salta, Sofia author Godinho, Maria Inês author Gómez, Antonio author Esteller, Manel author Costa, Bruno M. author Henrique, Rui author Jerónimo, Carmen 2018-07-27T10:56:36Z2018-07-27T10:56:36Z2018-03-272018-07-24T11:46:24Z Background: Prostate cancer (PCa) is a major cause of morbidity and mortality in men worldwide. MicroRNAs are globally downregulated in PCa, especially in poorly differentiated tumors. Nonetheless, the underlying mechanisms are still elusive. Herein, using combined analysis of microRNAs expression and genomewide DNA methylation, we aimed to identify epigenetically downregulated microRNAs in PCa. Results: We found that miR-152-3p was underexpressed in PCa and that lower expression levels were associated with promoter hypermethylation in accordance with TCGA dataset analysis. Functional in vitro assays suggest that miR-152-3p suppresses cell viability and invasion potential, whereas it promotes cell cycle arrest at S and G2/M phases. Additionally, miR-152-3p expression was associated with longer disease-free survival in PCa patients from TCGA. Finally, TMEM97, which is overexpressed in PCa, was identified as a novel miR-152-3p target gene. Conclusions: Our findings demonstrate the advantages of using a combinatory approach to identify microRNAs downregulated due to aberrant promoter methylation. MiR-152-3p downregulation and promoter methylation was found to be prevalent in primary PCa, which impairs its role in control of cell viability, cell cycle regulation and invasion. cc by (c) Ramalho-Carvalho et al., 2018 http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Càncer de pròstata ADN Prostate cancer DNA A multiplatform approach identifies miR-152-3p as a common epigenetically regulated onco-suppressor in prostate cancer targeting TMEM97 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion