2026-04-14T02:52:52Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1238392025-11-19T20:40:52Zcom_2072_1057col_2072_478781col_2072_478917

00925njm 22002777a 4500 dc Domínguez, Francisco author Simón, Carlos author Quiñonero, Alicia author Ramírez, Miguel Ángel author González Muñoz, Elena author Burghardt Coll, Hans author Cervero, Ana author Martínez, Sebastián author Pellicer, Antonio author Palacín Prieto, Manuel author Sánchez-Madrid, Francisco author Yáñez-Mó, María author 2018-07-24T09:21:42Z 2018-07-24T09:21:42Z 2010-10-15 2018-07-24T09:21:42Z Background Understanding the molecular basis of embryonic implantation is of great clinical and biological relevance. Little is currently known about the adhesion receptors that determine endometrial receptivity for embryonic implantation in humans. Methods and Principal Findings Using two human endometrial cell lines characterized by low and high receptivity, we identified the membrane receptor CD98 as a novel molecule selectively and significantly associated with the receptive phenotype. In human endometrial samples, CD98 was the only molecule studied whose expression was restricted to the implantation window in human endometrial tissue. CD98 expression was restricted to the apical surface and included in tetraspanin-enriched microdomains of primary endometrial epithelial cells, as demonstrated by the biochemical association between CD98 and tetraspanin CD9. CD98 expression was induced in vitro by treatment of primary endometrial epithelial cells with human chorionic gonadotropin, 17-β-estradiol, LIF or EGF. Endometrial overexpression of CD98 or tetraspanin CD9 greatly enhanced mouse blastocyst adhesion, while their siRNA-mediated depletion reduced the blastocyst adhesion rate. Conclusions These results indicate that CD98, a component of tetraspanin-enriched microdomains, appears to be an important determinant of human endometrial receptivity during the implantation window. Implantació de l'ou Endometri Ovum implantation Endometrium Human endometrial CD98 is essential for blastocyst adhesion