2026-04-13T05:42:35Zhttps://recercat.cat/oai/request
oai:recercat.cat:2445/1233572025-12-05T14:09:24Zcom_2072_1057col_2072_478815col_2072_478917col_2072_478921
RECERCAT author Ariza Sáenz, Martha Rocío author Espina García, Marta author Bolaños, Núria author Calpena Campmany, Ana Cristina author Gomara, Maria José author Haro, Isabel author García López, María Luisa 2018-07-04T13:17:50Z2018-12-31T06:10:25Z2017-112018-07-04T13:17:51Z Despite the great effort to decrease the HIV infectivity rate, current antiretroviral therapy has several weaknesses; poor bioavailability, development of drug resistance and poor ability to access tissues. However, molecules such as peptides have emerged as a new expectative to HIV eradication. The vaginal mucosa is the main spreading point of HIV. There are natural barriers such as the vaginal fluid which protects the vaginal epithelium from any foreign agents reaching it. This work has developed and characterized Nanoparticles (NPs) coated with glycol chitosan (GC), loaded with an HIV-1 inhibitor peptide (E2). In vitro release and ex vivo studies were carried out using the vaginal mucosa of swine and the peptide was determined by HPLC MS/MS validated method. Moreover, the peptide was labeled with 5(6)-carboxyfluoresceine and entrapped into the NPs to carried out in vivo studies and to evaluate the NPs penetration and toxicity in the vaginal mucosa of the swine. The mean size of the NPs, ξ and the loading percentage were fundamental features for to reach the vaginal tissue and to release the peptide within intercellular space. The obtained results suggesting that the fusion inhibitor peptides loaded into the NPs coated with GC might be a new way to fight the HIV-1, due to the formulation might reach the human epithelial mucosa and release peptide without any side effects. cc-by-nc-nd (c) Elsevier B.V., 2017 http://creativecommons.org/licenses/by-nc-nd/3.0/es info:eu-repo/semantics/openAccess Nanopartícules Pèptids Sistemes d'alliberament de medicaments Vagina Membrana mucosa Nanoparticles Peptides Drug delivery systems Vagina Mucous membrane Penetration of polymeric nanoparticles loaded with an HIV-1 inhibitor peptide derived from GB virus C in a vaginal mucosa model info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion