2026-04-13T03:03:54Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1227262025-12-05T14:23:38Zcom_2072_1057col_2072_478816col_2072_478917

00925njm 22002777a 4500 dc Cedó Giné, Lídia author Santos, David author Roglans i Ribas, Núria author Julve, Josep author Pallarès, Víctor author Rivas Urbina, Andrea author Llorente Cortés, Vicenta author Laguna Egea, Juan Carlos author Blanco-Vaca, Francisco author Escolà Gil, Joan Carles author 2018-06-01T13:42:35Z 2018-06-01T13:42:35Z 2017-12-15 2018-06-01T13:42:35Z Human hepatic lipase (hHL) is mainly localized on the hepatocyte cell surface where it hydrolyzes lipids from remnant lipoproteins and high density lipoproteins and promotes their hepatic selective uptake. Furthermore, hepatic lipase (HL) is closely associated with obesity in multiple studies. Therefore, HL may play a key role on lipid homeostasis in liver and white adipose tissue (WAT). In the present study, we aimed to evaluate the effects of hHL expression on hepatic and white adipose triglyceride metabolism in vivo. Experiments were carried out in hHL transgenic and wild-type mice fed a Western-type diet. Triglyceride metabolism studies included β-oxidation and de novo lipogenesis in liver and WAT, hepatic triglyceride secretion, and adipose lipoprotein lipase (LPL)-mediated free fatty acid (FFA) lipolysis and influx. The expression of hHL promoted hepatic triglyceride accumulation and de novo lipogenesis without affecting triglyceride secretion, and this was associated with an upregulation of Srebf1 as well as the main genes controlling the synthesis of fatty acids. Transgenic mice also exhibited more adiposity and an increased LPL-mediated FFA influx into the WAT without affecting glucose tolerance. Our results demonstrate that hHL promoted hepatic steatosis in mice mainly by upregulating de novo lipogenesis. HL also upregulated WAT LPL and promoted triglyceride-rich lipoprotein hydrolysis and adipose FFA uptake. These data support the important role of hHL in regulating hepatic lipid homeostasis and confirm the broad cardiometabolic role of HL. Obesitat Fetge Lipases Genètica Metabolisme dels lípids Obesity Liver Lipase Genetics Lipid metabolism Human hepatic lipase overexpression in mice induces hepatic statosis and obesity through promoting hepatic lipogenesis and chite adipose tissue lipolysisi and fatty acid uptake