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Coaggregation, cointernalization, and codesensitization of adenosine A2A receptors and dopamine D2 receptors Hillion, Joëlle Canals Buj, Meritxell Torvinen, Maria Casadó, Vicent Scott, Rizaldy Terasmaa, Anton Hansson, Anita Watson, Stanley Olah, Mark E. Mallol Montero, Josefa Canela Campos, Enric I. (Enric Isidre), 1949- Zoli, Michele Agnati, Luigi F. Ibanez, Carlos F. Lluís i Biset, Carme Franco Fernández, Rafael Ferré, Sergi Fuxe, Kjell Receptors cel·lulars Malalties del sistema nerviós Adenosina Cell receptors Nervous system Diseases Adenosine Antagonistic and reciprocal interactions are known to exist between adenosine and dopamine receptors in the striatum. In the present study, double immunofluorescence experiments with confocal laser microscopy showed a high degree of colocalization of adenosine A(2A) receptors (A(2A)R) and dopamine D(2) receptors (D(2)R) in cell membranes of SH-SY5Y human neuroblastoma cells stably transfected with human D(2)R and in cultured striatal cells. A(2A)R/D(2)R heteromeric complexes were demonstrated in coimmunoprecipitation experiments in membrane preparations from D(2)R-transfected SH-SY5Y cells and from mouse fibroblast Ltk(-) cells stably transfected with human D(2)R (long form) and transiently cotransfected with the A(2A)R double-tagged with hemagglutinin. Long term exposure to A(2A)R and D(2)R agonists in D(2)R-cotransfected SH-SY5Y cells resulted in coaggregation, cointernalization and codesensitization of A(2A)R and D(2)R. These results give a molecular basis for adenosine-dopamine antagonism at the membrane level and have implications for treatment of Parkinson's disease and schizophrenia, in which D(2)R are involved. 2018-05-11T13:59:15Z 2018-05-11T13:59:15Z 2002-05 2018-05-11T13:59:15Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 0021-9258 https://hdl.handle.net/2445/122309 501595 eng Reproducció del document publicat a: https://doi.org/10.1074/jbc.M107731200 Journal of Biological Chemistry, 2002, vol. 277, num. 20, p. 18091-18097 https://doi.org/10.1074/jbc.M107731200 (c) American Society for Biochemistry and Molecular Biology, 2002 info:eu-repo/semantics/openAccess 8 p. application/pdf American Society for Biochemistry and Molecular Biology Articles publicats en revistes (Bioquímica i Biomedicina Molecular)