2026-04-13T02:17:34Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1144842025-12-05T09:23:16Zcom_2072_1057col_2072_478799col_2072_478916col_2072_478917

00925njm 22002777a 4500 dc Silva-Martínez, Guillermo A. author Rodríguez-Ríos, Dalia author Alvarado-Caudillo, Yolanda author Vaquero García, Alejandro author Esteller, Manel author Carmona, F. Javier author Moran, Sebastian author Nielsen, Finn C. author Wickström-Lindholm, Marie author Wrobel, Katarzyna author Barbosa-Sabanero, Gloria author Zaina, Silvio author Lund, Gertrud author 2017-07-28T08:00:02Z 2017-07-28T08:00:02Z 2016-05-03 2017-07-28T08:00:02Z Abnormal fatty acid metabolism and availability are landmarks of metabolic diseases, which in turn are associated with aberrant DNA methylation profiles. To understand the role of fatty acids in disease epigenetics, we sought DNA methylation profiles specifically induced by arachidonic (AA) or oleic acid (OA) in cultured cells and compared those with published profiles of normal and diseased tissues. THP-1 monocytes were stimulated with AA or OA and analyzed using Infinium HumanMethylation450 BeadChip (Illumina) and Human Exon 1.0 ST array (Affymetrix). Data were corroborated in mouse embryonic fibroblasts. Comparisons with publicly available data were conducted by standard bioinformatics. AA and OA elicited a complex response marked by a general DNA hypermethylation and hypomethylation in the 1-200 μM range, respectively, with a maximal differential response at the 100 μM dose. The divergent response to AA and OA was prominent within the gene body of target genes, where it correlated positively with transcription. AA-induced DNA methylation profiles were similar to the corresponding profiles described for palmitic acid, atherosclerosis, diabetes, obesity, and autism, but relatively dissimilar from OA-induced profiles. Furthermore, human atherosclerosis grade-associated DNA methylation profiles were significantly enriched in AA-induced profiles. Biochemical evidence pointed to β-oxidation, PPAR-α, and sirtuin 1 as important mediators of AA-induced DNA methylation changes. In conclusion, AA and OA exert distinct effects on the DNA methylome. The observation that AA may contribute to shape the epigenome of important metabolic diseases, supports and expands current diet-based therapeutic and preventive efforts. Àcid araquidònic Àcid oleic Àcids grassos en la nutrició ADN Metilació Epigènesi Aterosclerosi Arachidonic acid Oleic acid Fatty acids in human nutrition DNA Methylation Epigenesis Atherosclerosis Arachidonic and oleic acid exert distinct effects on the DNA methylome