2026-04-13T14:24:38Zhttps://recercat.cat/oai/requestoai:recercat.cat:2445/1134422025-12-05T01:29:00Zcom_2072_1057col_2072_478917
Minimal genetic change in Vibrio cholerae in Mozambique over
time: Multilocus variable number tandem repeat analysis and
whole genome sequencing
Garrine, Marcelino
Mandomando, Inácio
Vubil, Delfino
Nhampossa, Tacilta
Acácio, Sozinho
Li, Shan
Paulson, Joseph N.
Almeida, Mathieu
Domman, Daryl
Thomson, Nicholas R.
Alonso, Pedro
Stine, Oscar C.
Còlera
Moçambic
Cholera
Mozambique
Although cholera is a major public health concern in Mozambique,
its transmission patterns remain unknown. We surveyed the
genetic relatedness of 75 Vibrio cholerae isolates from patients
at Manhica District Hospital between 2002-2012 and 3 isolates
from river using multilocus variable-number tandem-repeat
analysis (MLVA) and whole genome sequencing (WGS). MLVA revealed
22 genotypes in two clonal complexes and four unrelated
genotypes. WGS revealed i) the presence of recombination, ii) 67
isolates descended monophyletically from a single source
connected to Wave 3 of the Seventh Pandemic, and iii) four
clinical isolates lacking the cholera toxin gene. This Wave 3
strain persisted for at least eight years in either an
environmental reservoir or circulating within the human
population. Our data raises important questions related to where
these isolates persist and how identical isolates can be
collected years apart despite our understanding of high change
rate of MLVA loci and the V. cholerae molecular clock.
2017-07-06T12:24:57Z
2017-07-06T12:24:57Z
2017-06-16
2017-06-21T18:00:33Z
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Reproducció del document publicat a:
http://dx.doi.org/10.1371/journal.pntd.0005671
PLoS Neglected Tropical Diseases, 2017, vol. 11, num. 6, p.
e0005671
http://dx.doi.org/10.1371/journal.pntd.0005671
cc by (c) Garrine et al., 2017
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Public Library of Science
Articles publicats en revistes (ISGlobal)