2026-04-13T01:11:48Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1134422025-12-05T01:29:00Zcom_2072_1057col_2072_478917

00925njm 22002777a 4500 dc Garrine, Marcelino author Mandomando, Inácio author Vubil, Delfino author Nhampossa, Tacilta author Acácio, Sozinho author Li, Shan author Paulson, Joseph N. author Almeida, Mathieu author Domman, Daryl author Thomson, Nicholas R. author Alonso, Pedro author Stine, Oscar C. author 2017-07-06T12:24:57Z 2017-07-06T12:24:57Z 2017-06-16 2017-06-21T18:00:33Z Although cholera is a major public health concern in Mozambique, its transmission patterns remain unknown. We surveyed the genetic relatedness of 75 Vibrio cholerae isolates from patients at Manhica District Hospital between 2002-2012 and 3 isolates from river using multilocus variable-number tandem-repeat analysis (MLVA) and whole genome sequencing (WGS). MLVA revealed 22 genotypes in two clonal complexes and four unrelated genotypes. WGS revealed i) the presence of recombination, ii) 67 isolates descended monophyletically from a single source connected to Wave 3 of the Seventh Pandemic, and iii) four clinical isolates lacking the cholera toxin gene. This Wave 3 strain persisted for at least eight years in either an environmental reservoir or circulating within the human population. Our data raises important questions related to where these isolates persist and how identical isolates can be collected years apart despite our understanding of high change rate of MLVA loci and the V. cholerae molecular clock. Còlera Moçambic Cholera Mozambique Minimal genetic change in Vibrio cholerae in Mozambique over time: Multilocus variable number tandem repeat analysis and whole genome sequencing