2026-04-17T01:29:50Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1132452025-12-05T11:07:44Zcom_2072_1057col_2072_478780col_2072_478858col_2072_478917col_2072_478921

00925njm 22002777a 4500 dc Fenutría, Rafael author Martinez, Vanesa Gabriela author Simões, Inês author Postigo, Jorge author Gil, Victor author Martínez-Florensa, Mario author Sintes, Jordi author Naves, Rodrigo author Cashman, Kevin S. author Alberola-Ila, José author Ramos Casals, Manuel author Soldevila, Gloria author Raman, Chander author Merino, Jesús author Merino, Ramón author Engel Rocamora, Pablo author Lozano Soto, Francisco author 2017-07-03T11:23:15Z 2017-07-03T11:23:15Z 2014-01-15 2017-07-03T11:23:15Z CD5 is a lymphoid-specific transmembrane glycoprotein constitutively expressed on thymocytes and mature T and B1a lymphocytes. Current data support the view that CD5 is a negative regulator of antigen-specific receptor-mediated signaling in these cells, and that this would likely be achieved through interaction with CD5 ligand/s (CD5L) of still undefined nature expressed on immune or accessory cells. To determine the functional consequence of loss of CD5/CD5L interaction in vivo, a new transgenic mouse line was generated (shCD5EμTg), expressing a circulating soluble form of human CD5 (shCD5) as a decoy to impair membrane-bound CD5 function. These shCD5EμTg mice showed an enhanced response to autologous antigens, as deduced from the presentation of more severe forms of experimentally inducible autoimmune disease (collagen-induced arthritis, CIA; and experimental autoimmune encephalitis, EAE), as well as an increased anti-tumoral response in non-orthotopic cancer models (B16 melanoma). This enhancement of the immune response was in agreement with the finding of significantly reduced proportions of spleen and lymph node Treg cells (CD4+CD25+FoxP3+), and of peritoneal IL-10-producing and CD5+ B cells, as well as an increased proportion of spleen NKT cells in shCD5EμTg mice. Similar changes in lymphocyte subpopulations were observed in wild-type mice following repeated administration of exogenous recombinant shCD5 protein. These data reveal the relevant role played by CD5/CD5L interactions on the homeostasis of some functionally relevant lymphocyte subpopulations and the modulation of immune responses to autologous antigens. Cèl·lules B Cèl·lules T Limfòcits Antígens Melsa Resposta immunitària B cells T cells Lymphocytes Antigens Spleen Immune response Transgenic expression of soluble human CD5 enhances experimentally-induced autoimmune and anti-tumoral immune responses