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In vivo conditional deletion of HDAC7 reveals its requirement to establish proper B lymphocyte identity and development Azagra Rodríguez, Alba Román González, Lidia Collazo, Olga Rodríguez Ubreva, Javier Yébenes, Virginia G. de Barneda Zahonero, Bruna Rodríguez Lumbiarres, Jairo, 1980- Castro de Moura, Manuel Grego Bessa, Joaquim Fernández Duran, Irene Islam, Abul B. M. M. K. Esteller, Manel Ramiro, Almudena R. Ballestar Tarín, Esteban Parra Bola, Mª Isabel Cèl·lules B Histones Limfòcits Gens Proteïnes B cells Histones Lymphocytes Genes Proteins Class IIa histone deacetylase (HDAC) subfamily members are tissue-specific gene repressors with crucial roles in development and differentiation processes. A prominent example is HDAC7, a class IIa HDAC that shows a lymphoid-specific expression pattern within the hematopoietic system. In this study, we explored its potential role in B cell development by generating a conditional knockout mouse model. Our study demonstrates for the first time that HDAC7 deletion dramatically blocks early B cell development and gives rise to a severe lymphopenia in peripheral organs, while also leading to pro-B cell lineage promiscuity. We find that HDAC7 represses myeloid and T lymphocyte genes in B cell progenitors through interaction with myocyte enhancer factor 2C (MEFC2). In B cell progenitors, HDAC7 is recruited to promoters and enhancers of target genes, and its absence leads to increased enrichment of histone active marks. Our results prove that HDAC7 is a bona fide transcriptional repressor essential for B cell development. 2017-05-16T13:17:50Z 2017-05-30T22:01:30Z 2016-11 2017-05-16T13:17:50Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 0022-1007 https://hdl.handle.net/2445/111110 669675 27810920 eng Reproducció del document publicat a: https://doi.org/10.1084/jem.20150821 Journal of Experimental Medicine, 2016, vol. 213, num. 12, p. 2591-2601 https://doi.org/10.1084/jem.20150821 (c) Rockefeller University Press, 2016 info:eu-repo/semantics/openAccess 11 p. application/pdf Rockefeller University Press Articles publicats en revistes (Ciències Fisiològiques)