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MicroRNA expression profiling and DNA methylation signature for deregulated microRNA in cutaneous T-cell lymphoma Sandoval, Juan Diaz-Lagares, Angel Salgado, Rocío Servitje Bedate, Octavio Climent, Fina Ortiz Romero, Pablo Luis Pérez-Ferriols, Amparo Garcia-Muret, Maria P. Estrach Panella, Ma. Teresa (María Teresa) Garcia, Mar Nonell, Lara Esteller, Manel Pujol, Ramon M. Espinet Solà, Blanca Gallardo, F. (Fernando) Micro RNAs ADN Metilació Limfomes Cèl·lules T MicroRNAs DNA Methylation Lymphomas T cells MicroRNAs usually regulate gene expression negatively, and aberrant expression has been involved in the development of several types of cancers. Microarray profiling of microRNA expression was performed to define a microRNA signature in a series of mycosis fungoides tumor stage (MFt, n=21) and CD30+ primary cutaneous anaplastic large cell lymphoma (CD30+ cALCL, n=11) samples in comparison with inflammatory dermatoses (ID, n=5). Supervised clustering confirmed a distinctive microRNA profile for cutaneous T-cell lymphoma (CTCL) with respect to ID. A 40 microRNA signature was found in MFt including upregulated onco-microRNAs (miR-146a, miR-142-3p/5p, miR-21, miR-181a/b, and miR-155) and downregulated tumor-suppressor microRNAs (miR-200ab/429 cluster, miR-10b, miR-193b, miR-141/200c, and miR-23b/27b). Regarding CD30+ cALCL, 39 differentially expressed microRNAs were identified. Particularly, overexpression of miR-155, miR-21, or miR-142-3p/5p and downregulation of the miR-141/200c clusters were observed. DNA methylation in microRNA gene promoters, as expression regulatory mechanism for deregulated microRNAs, was analyzed using Infinium 450K array and approximately one-third of the differentially expressed microRNAs showed significant DNA methylation differences. Two different microRNA methylation signatures for MFt and CD30+ cALCL were found. Correlation analysis showed an inverse relationship for microRNA promoter methylation and microRNA expression. These results reveal a subgroup-specific epigenetically regulated microRNA signatures for MFt and CD30+ cALCL patients. 2017-05-15T12:56:26Z 2017-05-15T12:56:26Z 2015-04 2017-05-15T12:56:26Z info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Versió postprint del document publicat a: https://doi.org/10.1038/jid.2014.487 Journal of Investigative Dermatology, 2015, vol. 135, num. 4, p. 1128-1137 https://doi.org/10.1038/jid.2014.487 (c) Sandoval, Juan et al., 2015 info:eu-repo/semantics/openAccess Society for Investigative Dermatology Articles publicats en revistes (Ciències Fisiològiques)